PE Rabbit anti-Human/Monkey CD18 Monoclonal Antibody for Flow Cytometry - P05107

Core Product Specifications and Parameters

Biological Background: Integrin beta-2 Function and Localization

• Integrin beta-2 (CD18) forms heterodimeric receptors with alpha integrins (CD11a-d), binding ICAMs, iC3b, fibrinogen, and VCAM1, governing leukocyte adhesion and communication (PubMed:1676048, PubMed:23775590, PubMed:10438935, PubMed:8777714, PubMed:9841932, PubMed:38195629). Related references: PMID:1676048 | PMID:23775590 | PMID:10438935 | PMID:8777714 | PMID:9841932 | PMID:38195629
• This integrin mediates leukocyte transmigration and T-cell/neutrophil adhesion, essential for immune cell trafficking and inflammation (PubMed:11812992, PubMed:28807980, PubMed:18587400). Related references: PMID:11812992 | PMID:28807980 | PMID:18587400
• CD18 participates in phagocytic clearance via recognition of ICAM3 on apoptotic neutrophils and ICAM4 on erythrocytes, as well as natural killer cell cytotoxicity (PubMed:23775590, PubMed:16985175, PubMed:15356110). Related references: PMID:23775590 | PMID:16985175 | PMID:15356110
• The β2 integrin acts as a signaling receptor at the immunological synapse, where LFA-1–ICAM1 engagement couples TCR stimulation to adhesion strength (PubMed:38195629). Related references: PMID:38195629
• It is a transmembrane glycoprotein containing EGF-like domains, calcium/magnesium-binding sites, and is expressed on all leukocytes, with high levels on neutrophils; mutations cause leukocyte adhesion deficiency type 1.

Experimental Guidance and Technical Tips

• The immunogen spans the entire extracellular domain (aa 1-769) of human CD18, suggesting the antibody likely targets a surface-exposed epitope appropriate for live-cell flow cytometry.
• For flow cytometry, titrate the PE-conjugated antibody on relevant cell types (e.g., human PBMCs) to determine optimal staining concentration and minimize background.
• Include appropriate isotype control and live/dead discrimination to ensure specific detection of CD18+ leukocytes.
• Validate cross-reactivity with cynomolgus monkey by testing on PBMCs or isolated leukocytes from this species.
• Because CD18 is highly expressed on neutrophils, pre-block Fc receptors or use FcR blocking reagents to reduce non-specific binding.
• The PE label allows single-laser detection; ensure compensation settings if multiplexing with other fluorochromes.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with one-stop access to IVD raw materials, including validated monoclonal antibodies like this anti-CD18 clone, optimized for flow cytometry. Beyond antibodies, we offer bulk ancillary reagents and sourcing services for rare targets, streamlining your development from concept to clinic. Contact us to explore antibody pairs, technical support, and custom sourcing solutions for leukocyte adhesion and immunology research.