PE Rabbit anti-Human/Monkey CD16 Monoclonal Antibody for FC - P08637/O75015

Core Product Specifications and Parameters

Biological Background: CD16 Function and Localization

• CD16, also known as FCGR3, exists as two isoforms: FCGR3A (CD16a) and FCGR3B (CD16b), encoded by distinct genes (FCGR3A and FCGR3B) that share high sequence homology and mediate IgG Fc fragment binding. Related references: PMID:11711607 PMID:21768335 PMID:22023369

• CD16a, expressed on NK cells and monocytes, triggers antibody-dependent cellular cytotoxicity (ADCC) upon engaging clustered antigen-IgG complexes, leading to target cell lysis and cytokine production, which is crucial for antiviral and antitumor immunity. Related references: PMID:24412922 PMID:25786175 PMID:29967280 PMID:9916693

• Signaling through CD16a involves phosphorylation of ITAM-containing adapters, activating PI3K and calcium flux pathways; this leads to both degranulation and pro-inflammatory cytokine production, with distinct signaling outcomes depending on receptor phosphorylation status. Related references: PMID:1825220 PMID:23024279 PMID:2532305

• CD16a plays a pivotal role in the mechanism of therapeutic monoclonal antibodies, enhancing ADCC against tumor cells; afucosylated IgGs exhibit higher affinity for CD16a, resulting in more potent effector functions. Related references: PMID:27670158 PMID:34485821 PMID:28566370

• During secondary Dengue virus infection, CD16a can mediate antibody-dependent enhancement (ADE) by facilitating viral entry into myeloid cells via afucosylated IgG1-virus immune complexes, exacerbating pathogenesis.

• CD16a is a transmembrane glycoprotein localized predominantly on the cell membrane of NK cells and a subset of circulating monocytes; a soluble secreted form is also generated, contributing to its diverse functions.

• The protein is characterized by immunoglobulin-like domains, disulfide bonds, and N-linked glycosylation, which are essential for its structural integrity and ligand binding; phosphorylation further modulates its signaling capacity.

Experimental Guidance and Technical Tips

• For flow cytometry, titrate the PE-conjugated antibody to determine the optimal concentration for your specific cell type and experimental conditions, using human PBMCs or cynomolgus monkey samples as positive controls.
• Include appropriate isotype controls and Fc blocking steps to minimize non-specific binding, especially when analyzing Fc receptor-expressing cells.
• The PE fluorophore is bright and suitable for low-abundance target detection; consider its compatibility with other fluorophores when designing multicolor panels and ensure proper compensation.
• Validate the antibody’s performance in your intended application, such as ADCC reporter assays or immune cell profiling, using relevant biological replicates and activation states.
• This antibody may also be applicable for studying CD16 isoforms in functional assays; cross-reactivity with cynomolgus supports translational research models.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research labs comprehensive access to IVD raw materials, including validated antibody pairs, bulk ancillary reagents, and specialized monoclonal antibodies like this anti-CD16 clone. Our one-stop sourcing platform simplifies procurement of critical reagents for immune profiling and ADCC assay development, from concept to clinic. For custom requirements or rare target sourcing, our technical team ensures seamless supply chain support tailored to your workflow.