PE Rabbit anti-Human/Monkey CD14 Monoclonal Antibody for Flow Cytometry - P08571

Core Product Specifications and Parameters

Biological Background: CD14 Function and Localization

• CD14, also known as monocyte differentiation antigen, My23 antigen, or myeloid cell-specific leucine-rich glycoprotein, is a key player in innate immunity.
• It acts as a coreceptor for bacterial lipopolysaccharide (LPS), binding monomeric LPS in concert with LBP and delivering it to the LY96/TLR4 complex, thereby mediating the innate immune response to bacterial LPS. Related references: PMID:1698311 PMID:23264655 PMID:20133493 PMID:22265692
• Downstream signaling occurs via MyD88, TIRAP, and TRAF6, leading to NF-kappa-B activation, cytokine secretion, and the inflammatory response. Related references: PMID:8612135
• CD14 also functions as a coreceptor for TLR2:TLR6 and TLR2:TLR1 heterodimers in response to diacylated and triacylated lipopeptides; these clusters trigger cell surface signaling and subsequent lipid-raft-dependent trafficking to the Golgi. Related references: PMID:16880211
• Additionally, CD14 binds electronegative LDL (LDL(-)) and mediates cytokine release induced by LDL(-), linking innate immunity to lipid metabolism. Related references: PMID:23880187
• Subcellularly, CD14 is located on the cell membrane, membrane rafts, and Golgi apparatus, and is also found as a secreted protein. It is a leucine-rich repeat (LRR) glycoprotein with a GPI-anchor.
• Tissue specificity: CD14 is strongly expressed on the surface of monocytes and weakly on granulocytes; it is detected on macrophages and most tissue macrophages. Related references: PMID:1698311

Experimental Guidance and Technical Tips

• The immunogen comprises the extracellular domain (amino acids 20–352) of human CD14, suggesting the antibody is likely to recognize the native conformation of the receptor. Validate staining in your relevant sample system using appropriate positive and negative controls.
• For flow cytometry, titrate the PE-conjugated antibody to determine the optimal working concentration for your cell type of interest. Use an isotype control to assess nonspecific binding.
• Cross-reactivity with cynomolgus monkey CD14 has been demonstrated; if using other species, verify reactivity independently.
• Avoid prolonged light exposure when working with the PE conjugate to maintain signal integrity.

CamelBio: Your One-Stop Sourcing Bridge

At CamelBio, we serve as your comprehensive bridge for IVD raw material sourcing—from concept to clinic. For CD14 and innate immunity research, our portfolio includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets. Partner with us to accelerate your diagnostic assay development with reliable, one-stop support.