PE Rabbit anti-Human/Monkey CD127/IL-7R Monoclonal Antibody for Flow Cytometry - P16871

Core Product Specifications and Parameters

Biological Background: CD127/IL-7R Function and Localization

• CD127 (IL-7R alpha) is the alpha subunit of the interleukin-7 receptor, which also associates with the common gamma chain (γc) to form a functional receptor for IL-7.
• Additionally, it functions as a receptor for thymic stromal lymphopoietin (TSLP) when paired with the TSLP receptor (TSLPR).
• The protein exists as both a membrane-bound form and a soluble form (sIL-7R) generated by alternative splicing, with the latter playing a regulatory role.
• Subcellular localization includes the cell membrane (via a transmembrane domain) and secretion of the soluble isoform.
• Post-translational modifications include N-linked glycosylation, phosphorylation, and ubiquitination, which modulate receptor stability and signaling.
• Mutations in the IL7R gene are associated with severe combined immunodeficiency (SCID), highlighting its critical role in lymphocyte development and immune homeostasis.
• The molecular weight of the mature glycosylated protein is approximately 52 kDa, consistent with both UniProt data (51.6 kDa) and experimental observations.

Experimental Guidance and Technical Tips

• This PE-conjugated antibody is specifically intended for flow cytometry applications; no other applications are listed, so cross-application validation is recommended if needed.
• Given the immunogen spans the extracellular domain (aa 21-236), the antibody likely recognizes an epitope accessible on live cells, suitable for surface staining.
• Include appropriate isotype controls and Fc receptor blocking steps to minimize non-specific binding.
• Validate the staining protocol on known CD127-positive cell types (e.g., human peripheral blood T cells, regulatory T cells) to confirm reactivity in your experimental system.
• The cross-reactivity with cynomolgus monkey enables translational research and preclinical testing. Verify performance in monkey samples with an appropriate secondary reagent if required.

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