PE Rabbit anti-Human TROP-2 Monoclonal Antibody for Flow Cytometry - P09758

Core Product Specifications and Parameters

Biological Background: TROP-2 Function and Localization

• TROP-2 (Tumor-associated calcium signal transducer 2) is encoded by the TACSTD2 gene and is also known as cell surface glycoprotein Trop-2, EGP1, GP50, M1S1, and pancreatic carcinoma marker protein GA733-1.
• It may function as a growth factor receptor, although its downstream signaling pathways remain to be fully characterized.
• TROP-2 is a transmembrane glycoprotein localized to the cell membrane.
• Expression is reported in placenta and pancreatic carcinoma cell lines, highlighting its role as a tumor-associated antigen.
• The protein contains a signal peptide, a large extracellular domain, a single transmembrane helix, and a short cytoplasmic tail.
• Post-translational modifications include N-linked glycosylation and a disulfide bond, contributing to its structural integrity.
• UniProt annotations link TROP-2 to disease conditions such as amyloidosis and corneal dystrophy, though its involvement requires further investigation.
• As a tumor antigen, TROP-2 is an attractive target for cancer immunotherapy and diagnostic marker development.

Experimental Guidance and Technical Tips

• Since the immunogen corresponds to the extracellular domain (aa 27-274), the antibody likely binds to surface-expressed TROP-2, making it suitable for flow cytometry with live or fixed cells.
• For optimal staining, titrate the PE-conjugated antibody in the relevant experimental system to determine the working dilution.
• Include appropriate isotype controls and use known TROP-2-positive and -negative cell lines (e.g., pancreatic carcinoma vs. normal tissue) to confirm specificity.
• When designing multicolor panels, consider PE's bright emission and fluorescence compensation requirements, especially when co-staining with other fluorophores.
• Avoid prolonged light exposure of PE-labeled antibodies to prevent photobleaching.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing bridge for IVD raw materials, offering monoclonal antibodies, antibody pairs, and ancillary reagents to accelerate your diagnostic and research workflows. Our portfolio includes PE-conjugated anti-TROP-2 antibodies suitable for tumor marker studies, complemented by bulk supply options and tailored sourcing for rare or niche targets. From early-stage concept to clinical application, CamelBio provides technical support and reliable procurement to streamline your assay development.