PE Rabbit anti-Human TMPRSS2 Monoclonal Antibody for Flow Cytometry - O15393

Core Product Specifications and Parameters

Biological Background: TMPRSS2 Function and Localization

• TMPRSS2 (Transmembrane protease serine 2, PRSS10) is a plasma membrane-anchored serine protease that cleaves at arginine residues, functioning as a key enzyme in proteolytic cascades relevant to prostate physiology (PubMed:32703818, PubMed:35676539, PubMed:37990007). Related references: PMID:32703818 PMID:35676539 PMID:37990007
• Androgen-induced TMPRSS2 activates substrates such as pro-HGF, F2RL1, and matriptase/ST14, promoting extracellular matrix disruption and metastasis in prostate cancer cells (PubMed:15537383, PubMed:25122198, PubMed:26018085). Related references: PMID:15537383 PMID:25122198 PMID:26018085
• Facilitates infections by human coronaviruses SARS-CoV and SARS-CoV-2 via two independent mechanisms: proteolytic cleavage of ACE2 receptor and cleavage of coronavirus spike glycoproteins, which activates the glycoprotein for host cell entry (PubMed:24227843, PubMed:32142651, PubMed:32404436, PubMed:32703818, PubMed:33051876, PubMed:34159616, PubMed:35676539). Related references: PMID:24227843 PMID:32142651 PMID:32404436 PMID:32703818 PMID:33051876 PMID:34159616 PMID:35676539
• Also cleaves and activates spike glycoproteins of other coronaviruses (HCoV-229E, HCoV-EMC) and fusion glycoproteins of Sendai virus, human metapneumovirus, and parainfluenza viruses; essential for influenza A virus spread via hemagglutinin activation.
• Acts as a receptor for human coronavirus HKU1-CoV, synergizing with disialoside glycans to facilitate viral entry, and its interaction with spike protein inhibits TMPRSS2 protease activity.
• Localizes to the cell membrane and is also secreted; contains a signal-anchor and a transmembrane helix.
• Highly expressed in epithelial cell-rich tissues, with highest levels in prostate gland, and also in lung type II pneumocytes, small intestine, colon, stomach, and salivary gland; coexpressed with ACE2 in several tissues.
• Key post-translational modifications include disulfide bond formation and glycosylation, and it is synthesized as a zymogen requiring autocatalytic cleavage for activation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the serine protease domain (amino acids 106-255) within the extracellular region of TMPRSS2; this PE-conjugated antibody is designed for flow cytometry detection of cell surface TMPRSS2 on live, unfixed cells.
• For optimal results, validate in cell lines or primary samples with confirmed TMPRSS2 expression, such as prostate cancer (LNCaP) or lung epithelial cells.
• Include appropriate isotype controls and consider Fc receptor blocking to minimize non-specific binding.
• Perform titration experiments to determine the optimal antibody concentration for your specific experimental setup.
• Note that TMPRSS2 expression may be influenced by androgen levels; consider stimulation conditions if expression is low.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with comprehensive access to IVD raw materials, from validated antibody pairs and optimized monoclonal/polyclonal antibodies to bulk ancillary reagents and rare target sourcing. This PE Rabbit anti-Human TMPRSS2 mAb supports research in oncology and infectious disease, and CamelBio's supply chain capabilities can accommodate large-scale production and customized procurement to accelerate your assay development.