PE Rabbit anti-Human TLR1 Monoclonal Antibody for FC - Q15399

Core Product Specifications and Parameters

Biological Background: TLR1 Function and Localization

• TLR1, also known as CD281 and Toll/interleukin-1 receptor-like protein (TIL), is a type I transmembrane receptor belonging to the Toll-like receptor family. It is a key component of the innate immune system, responsible for recognizing pathogen-associated molecular patterns.
• TLR1 specifically recognizes diacylated and triacylated lipopeptides and cooperates with TLR2 to mediate the innate immune response to bacterial lipoproteins or lipopeptides. Related references: PMID:21078852
• Upon ligand recognition, TLR1 forms an activation cluster with TLR2 and CD14 on the cell surface. This complex initiates signaling from the cell membrane and subsequently traffics to the Golgi apparatus via a lipid-raft dependent pathway. Related references: PMID:16880211
• Downstream signaling proceeds through the adaptor proteins MYD88 and TRAF6, leading to NF-kappa-B activation, pro-inflammatory cytokine secretion, and the inflammatory response.
• Subcellular localization is dynamic: TLR1 is found at the cell membrane, within cytoplasmic vesicles, on phagosome membranes, in membrane rafts, and at the Golgi apparatus, reflecting its trafficking after activation.
• TLR1 is ubiquitously expressed, with notably high levels in spleen, ovary, peripheral blood leukocytes, thymus, and small intestine.
• As a glycoprotein, TLR1 contains extracellular leucine-rich repeats (LRRs) for ligand recognition, a single transmembrane helix, and an intracellular Toll/interleukin-1 receptor (TIR) domain essential for signal transduction.

Experimental Guidance and Technical Tips

• The immunogen used to generate this antibody is a synthetic peptide corresponding to amino acids 325-424 within the extracellular domain of human TLR1. This region is likely accessible on the cell surface, supporting its use in flow cytometry.
• For flow cytometry, titrate the antibody to determine the optimal staining concentration for your specific cell type and experimental conditions.
• Since TLR1 can be expressed on both the cell surface and in intracellular compartments, consider surface staining for live cells and permeabilization for total TLR1 detection if intracellular pools are of interest.
• Use an appropriate isotype control antibody and viability dye to assess and minimize non-specific binding and background.
• The PE conjugate allows direct detection; ensure proper compensation when combining with other fluorophores in multicolor panels.
• Cross-reactivity with species other than human has not been evaluated; validate the antibody if used with non-human samples.

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CamelBio offers a comprehensive range of IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing services. Whether you require antibodies for innate immunity targets like TLR1 or broader diagnostic development needs, our team provides tailored procurement support from concept to clinic. Explore our catalog for integrated solutions to accelerate your assay development.