PE Rabbit anti-Human TIM-3/HAVCR2 Monoclonal Antibody for FC - Q8TDQ0

Core Product Specifications and Parameters

Biological Background: TIM-3/HAVCR2 Function and Localization

• TIM-3 is a coinhibitory receptor on T cells, attenuating TCR signaling and inhibiting Th1 responses, promoting immunological tolerance. Related references: PMID:14556005

• It binds to galectin-9 (LGALS9), leading to suppression of T-cell responses and apoptosis of antigen-specific cells. Related references: PMID:16286920 PMID:24337741

• TIM-3 also recognizes phosphatidylserine on apoptotic cells, mediating phagocytosis by dendritic cells.

• On natural killer (NK) cells, TIM-3 acts as a coreceptor to enhance IFN-gamma production in response to LGALS9, but can also suppress NK cell cytotoxicity. Related references: PMID:22323453 PMID:22383801

• Expressed on regulatory T cells, TIM-3 inhibits Th17 cell responses, contributing to immune homeostasis. Related references: PMID:24838857

• TIM-3 expression is upregulated on exhausted T cells in chronic viral infections like HCV and HIV, and in tumor microenvironments, marking it as a key immune checkpoint.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (aa 22-200), which includes the ligand-binding region; this design supports detection of native surface-expressed TIM-3 in flow cytometry.
• For FC, consider using this PE-conjugated antibody for direct staining of human PBMCs or isolated T-cell subsets; validate in the relevant sample system to confirm expected expression patterns.
• Note that TIM-3 expression can be induced upon T-cell activation; appropriate stimulation controls may be required for accurate gating.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and labs with one-stop access to high-quality IVD raw materials. This PE-conjugated anti-TIM-3 antibody supports immune checkpoint research and clinical flow cytometry applications. We offer validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents to streamline your assay development and production needs.