PE Rabbit anti-Human Tie-2/CD202b Monoclonal Antibody for Flow Cytometry - Q02763

Core Product Specifications and Parameters

Biological Background: Tie-2/CD202b Function and Localization

• Tie-2 (TEK, CD202B) is a receptor tyrosine kinase that serves as the cell-surface receptor for angiopoietins (ANGPT1, ANGPT2, ANGPT4), playing a central role in angiogenesis and vascular homeostasis.
• It regulates endothelial cell survival, proliferation, migration, adhesion, and cytoskeletal reorganization; depending on cellular context, it can either promote vascular quiescence or stimulate sprouting angiogenesis.
• In quiescent vessels, ANGPT1-induced Tie-2 clustering at cell-cell junctions activates AKT1 signaling to stabilize vessels, while in migrating cells lacking junctions, ANGPT1 recruits Tie-2 to focal adhesions, activating FAK and MAPK pathways to support angiogenesis.
• Tie-2 is predominantly expressed in endothelial cells and their progenitors, with high levels in placenta and lung, and lower expression in brain and kidney; a soluble extracellular fragment is detectable in plasma.
• The protein localizes not only to the cell membrane but also to cell junctions, focal adhesions, cytoplasm, and cytoskeleton, reflecting its dynamic role in signaling and cell adhesion.
• Tie-2 undergoes proteolytic processing to release a soluble extracellular domain that acts as a decoy receptor for angiopoietins, modulating signaling.
• Mutations in the TEK gene are associated with vascular malformations (e.g., venous malformations, multiple cutaneous and mucosal, VMCM) and primary congenital glaucoma (GLC3E), highlighting its critical role in vascular development and eye physiology.
• Post-translational modifications include N-linked glycosylation, disulfide bond formation, and phosphorylation on tyrosine residues upon ligand binding.

Experimental Guidance and Technical Tips

• This antibody is a PE-conjugated monoclonal antibody validated for flow cytometry on human samples; it recognizes the extracellular domain of Tie-2 (aa 23-745), making it suitable for detecting cell surface expression on live, non-permeabilized cells.
• For optimal FC staining, consider titrating the antibody to determine the saturating concentration and include appropriate isotype controls and Fc receptor blocking.
• The antibody cross-reacts with human Tie-2; for other species, check sequence homology in the immunogen region to predict reactivity.
• Since Tie-2 is expressed at higher levels on endothelial cells, use relevant positive controls such as HUVECs or endothelial progenitor cells.
• Avoid extended storage at room temperature; the antibody is shipped on ice bags and should be stored at 2-8°C protected from light.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with a single-channel sourcing hub for IVD raw materials, including this high-quality anti-Tie-2 monoclonal antibody. Beyond this PE-conjugated reagent, we offer a range of unlabeled and validated antibody pairs, bulk ancillary reagents, and customized solutions to support your angiogenesis and vascular biology assay development from concept to clinic.