PE Rabbit anti-Human STAT1 Monoclonal Antibody for Flow Cytometry - P42224

Core Product Specifications and Parameters

Biological Background: STAT1 Function and Localization

• STAT1 is a key signal transducer and activator of transcription that mediates cellular responses to interferons (IFNs), KITLG/SCF, and other cytokines and growth factors. Related references: PMID:12764129, PMID:12855578, PMID:15322115
• Following type I IFN binding, STAT1 is phosphorylated, forms the ISGF3 complex with STAT2 and IRF-9, translocates to the nucleus, and binds ISRE to drive antiviral gene expression. Related references: PMID:28753426, PMID:35568036
• In response to type II IFN (IFN-gamma), STAT1 forms a homodimer (GAF) that binds GAS elements to activate target genes, inducing a cellular antiviral state. Related references: PMID:8156998
• STAT1 is tyrosine- and serine-phosphorylated upon activation; phosphorylation at Thr-749 by IKBKB promotes transcription of ARID5A and IL12B. Related references: PMID:26479788, PMID:32209697
• STAT1 mediates responses to activated FGFR family members and has roles in food tolerance in the small intestine via association with Gasdermin-D p13 fragment and promotion of CIITA transcription in Tr1 cells.
• Subcellular localization is primarily cytoplasmic, with nuclear translocation upon activation.
• The protein undergoes various post-translational modifications including acetylation, ADP-ribosylation, methylation, and ubiquitination; classified under keywords such as antiviral defense, host-virus interaction, and disease variant.

Experimental Guidance and Technical Tips

• The immunogen spans the C-terminal region (aa 650–750) of human STAT1, which includes key regulatory sites. The antibody is expected to recognize total STAT1 irrespective of phosphorylation status.
• For intracellular flow cytometry (FC intra), optimize fixation and permeabilization protocols to ensure access to nuclear STAT1. Titrate the antibody to achieve optimal signal-to-noise ratios.
• Validate the antibody in your specific sample system, considering that STAT1 expression can be upregulated by interferons and other stimuli. Use appropriate positive (e.g., IFN-treated cells) and negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a dedicated B2B sourcing partner for diagnostic manufacturers and research institutions, offering one-stop access to IVD raw materials. Our portfolio includes validated anti-STAT1 antibodies, recombinant proteins, and ancillary reagents tailored for antiviral and immune signaling research. We leverage global supply networks to source rare-target raw materials and provide technical support from development to commercialization.