PE Rabbit anti-Human Siglec-8 Monoclonal Antibody for FC - Q9NYZ4

Core Product Specifications and Parameters

Biological Background: Siglec-8 Function and Localization

• Siglec-8, also known as SAF-2 (sialoadhesin family member 2), is a single-pass type I membrane glycoprotein belonging to the immunoglobulin superfamily. Alternative splicing generates multiple isoforms.
• Siglec-8 mediates sialic-acid dependent adhesion to blood cells, preferentially binding α-2,3-linked sialic acid with some affinity for α-2,6-linkages; cis interactions on the same cell surface may mask the ligand-binding site. Related references: PMID:10625619, PMID:10856141
• It specifically recognizes terminal N-acetylneuraminic acid (sialic acid) and underlying 6-O-sulfated galactose, with a strong preference for Gal-6-sulfated sialyl-Lewis X glycan epitopes. Related references: PMID:27357658
• The protein is predominantly expressed on the surface of eosinophils, basophils, and mast cells, making it a specific marker for these cell types in hematopoietic studies.
• Subcellular localization is primarily at the plasma membrane, consistent with its role in cell-cell interactions and signaling.
• Siglec-8 contains immunoglobulin-like domains and disulfide bonds, typical of adhesion molecules, and is post-translationally modified by glycosylation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (amino acids 17-363), suggesting the antibody likely recognizes surface-exposed epitopes, making it suitable for flow cytometry on live, non-permeabilized cells.
• For flow cytometry applications, titrate the PE-conjugated antibody to determine the optimal staining concentration using relevant cell lines or primary cells known to express Siglec-8 (e.g., eosinophil, basophil, or mast cell populations).
• Include appropriate isotype controls and Fc receptor blocking steps to minimize non-specific binding.
• Validate staining specificity in the intended sample system, considering the reported expression of Siglec-8 on human basophils, eosinophils, and mast cells.
• Note that multiple protein isoforms exist (44, 46, 54 kDa), but the PE conjugate is designed for cell surface detection and will not distinguish molecular weight variants.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides comprehensive IVD raw-material solutions, offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, and ancillary reagents to support your diagnostic and research workflows. This PE-conjugated anti-Siglec-8 antibody exemplifies our capability to deliver specialized immunoreagents for flow cytometric analysis, with a focus on rare and emerging targets in immunology and allergy research. Leverage our one-stop sourcing platform to secure high-quality raw materials and accelerate your development from concept to clinic.