PE Rabbit anti-Human Siglec-7/CD328 Monoclonal Antibody for FC - Q9Y286

Core Product Specifications and Parameters

Biological Background: Siglec-7 Function and Localization

• Sialic acid-binding Ig-like lectin 7 (Siglec-7), also known as CD328, AIRM-1, p75, and D-siglec, is encoded by the SIGLEC7 gene. It is a single-pass type I membrane protein belonging to the immunoglobulin superfamily.
• Functions as a putative adhesion molecule that mediates sialic-acid dependent binding to cells, with preference for α-2,3- and α-2,6-linked sialic acids and disialogangliosides. The sialic acid recognition site may be masked by cis interactions on the same cell surface.
• Acts as an inhibitory receptor in the immune response: upon ligand engagement, it undergoes tyrosine phosphorylation and recruits cytoplasmic phosphatases (e.g., SHP-1, SHP-2) via SH2 domains, leading to dephosphorylation of signaling molecules and attenuation of immune cell activation.
• Mediates inhibition of natural killer (NK) cell cytotoxicity, playing a critical role in regulating NK cell function and innate immunity.
• Involved in hematopoiesis; has been shown to inhibit differentiation of CD34+ cell precursors toward the myelomonocytic lineage and suppress proliferation of leukemic myeloid cells in vitro.
• Predominantly expressed on resting and activated NK cells, with lower levels on granulocytes and monocytes. High expression is observed in placenta, liver, lung, spleen, and peripheral blood leukocytes.
• Post-translational modifications include glycosylation and phosphorylation. Contains a signal peptide, an extracellular region with two Ig-like domains (one V-set, one C2-set), a transmembrane helix, and a cytoplasmic tail with ITIM and ITIM-like motifs.

Experimental Guidance and Technical Tips

• Since the immunogen corresponds to the extracellular domain (aa 18-144), the antibody likely recognizes an epitope in the N-terminal region, suitable for detecting native surface Siglec-7.
• This PE-conjugated antibody is intended for direct flow cytometry; optimal working dilution should be determined experimentally by titration in the relevant cell system (e.g., human PBMCs or NK cell lines).
• Consider including appropriate isotype controls and Fc receptor blocking to minimize non-specific binding.
• For combined multicolor flow cytometry panels, verify that the PE conjugate is compatible with other fluorophores and compensate appropriately.

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated IVD raw material partner, CamelBio offers a streamlined sourcing experience for diagnostic developers and researchers. This anti-Siglec-7 antibody serves researchers in immunology and immuno-oncology, supporting studies on NK cell regulation and immune checkpoints. CamelBio provides validated antibody pairs, bulk monoclonal and polyclonal antibodies, and ancillary reagents, along with customized sourcing for rare targets.