PE Rabbit anti-Human PTH1R Monoclonal Antibody for FC - Q03431

Core Product Specifications and Parameters

Biological Background: PTH1R Function and Localization

• PTH1R is a G protein-coupled receptor that serves as the receptor for parathyroid hormone (PTH) and parathyroid hormone-related peptide (PTHLH) (PubMed:10913300, PubMed:18375760, PubMed:19674967). Related references: PMID:10913300 PMID:18375760 PMID:19674967
• Ligand binding induces a conformational change, triggering signaling through G proteins and modulation of downstream effectors such as adenylate cyclase, leading to cAMP production (PubMed:30975883, PubMed:35932760). Related references: PMID:30975883 PMID:35932760
• PTH1R couples to G(s) alpha proteins, mediating the activation of adenylate cyclase activity (PubMed:20172855, PubMed:30975883, PubMed:35932760). Related references: PMID:20172855 PMID:30975883 PMID:35932760
• PTHLH dissociates from PTH1R more rapidly than PTH, resulting in a faster decay of cAMP response (PubMed:35932760). Related references: PMID:35932760
• PTH1R is localized at the cell membrane, consistent with its role as a transmembrane receptor.
• The receptor is expressed in most tissues, with highest abundance in kidney, bone, and liver.
• Post-translational modifications include glycosylation, phosphorylation, and disulfide bonds, as indicated by UniProt keywords.
• Genetic variants of PTH1R are associated with dwarfism, highlighting its role in skeletal development.

Experimental Guidance and Technical Tips

• The immunogen used to generate this antibody corresponds to amino acids 27–188 of human PTH1R, which comprises the N-terminal extracellular domain. This design may be suitable for detecting the native receptor on the cell surface.
• As this antibody is PE-conjugated, it is ready-to-use for direct flow cytometry (FC) detection of PTH1R expressed on live or fixed cells. Consider titrating the antibody for optimal staining.
• Validate the antibody in your specific sample system, as PTH1R expression levels can vary across cell types and tissues.

CamelBio: Your One-Stop Sourcing Bridge

As a one-stop IVD raw-material sourcing partner, CamelBio supports diagnostic manufacturers and research teams with high-quality antibodies and reagents for GPCR targets like PTH1R. We offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and can assist in sourcing rare target raw materials, ensuring seamless procurement from discovery to clinical application.