PE Rabbit anti-Human PLZF Monoclonal Antibody for FC (intra) - Q05516

Core Product Specifications and Parameters

Biological Background: PLZF Function and Localization

• Zinc finger and BTB domain-containing protein 16 (ZBTB16), also known as promyelocytic leukemia zinc finger protein (PLZF) and zinc finger protein 145 (ZNF145).
• Acts as a transcriptional repressor, with repression likely mediated through recruitment of histone deacetylases to target promoters. Related references: PMID:10688654 | PMID:24359566
• Serves as a probable substrate-recognition component of an E3 ubiquitin-protein ligase complex, directing ubiquitination and proteasomal degradation of target proteins. Related references: PMID:14528312
• Implicated in myeloid maturation and the development and/or maintenance of other differentiated tissues.
• Predominantly localizes to the nucleus and nuclear bodies.
• Highly expressed in the hematopoietic system: bone marrow, early myeloid cell lines, and peripheral blood mononuclear cells; also found in ovary, and at lower levels in kidney and lung.
• Contains classical C2H2-type zinc finger domains and a BTB/POZ domain; subject to alternative splicing, phosphorylation, and ubiquitination; clinically associated with chromosomal rearrangements in leukemia and intellectual disability.

Experimental Guidance and Technical Tips

• For intracellular flow cytometry, optimize fixation and permeabilization conditions to maintain epitope accessibility; titrate the antibody to determine the optimal staining concentration.
• Validate antibody performance in a relevant human hematopoietic cell line known to express PLZF (e.g., NB4 or HL-60) before testing on primary samples.
• Include appropriate isotype controls and consider careful fluorophore compatibility when designing multi-color panels.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, offering validated antibodies, recombinant proteins, and ancillary reagents to support diagnostic development from concept to clinic. Our portfolio includes this PE-conjugated anti-PLZF monoclonal antibody, ideal for intracellular flow cytometry in hematopoietic research and transcription factor analysis. We provide end-to-end technical services and rapid sourcing of rare targets to accelerate your assay development.