PE Rabbit anti-Human Phospho-Histone H2AX-S139 Monoclonal Antibody for Flow Cytometry - P16104

Core Product Specifications and Parameters

Biological Background: Histone H2AX Function and Localization

• Histone H2AX is a variant histone H2A that replaces conventional H2A in a subset of nucleosomes.
• It plays a central role in transcription regulation, DNA repair, DNA replication, and chromosomal stability by compacting DNA into chromatin.
• H2AX is critical for the DNA damage response; upon double-strand breaks, it is rapidly phosphorylated at Ser139 (γ-H2AX), forming foci that recruit repair proteins.
• It is required for checkpoint-mediated cell cycle arrest in response to low doses of ionizing radiation.
• Subcellular localization is nuclear, specifically on chromosomes.
• Post-translational modifications include phosphorylation, acetylation, and ubiquitination, which regulate its function in DNA repair and chromatin dynamics.
• May also have roles in meiosis and host-virus interactions.

Experimental Guidance and Technical Tips

• Since the antibody targets a phosphorylation site, consider including phosphatase inhibitors during cell preparation and staining.
• For intracellular flow cytometry, appropriate fixation and permeabilization buffers should be optimized to preserve the phospho-epitope.
• The PE conjugation allows direct detection; avoid using PE-conjugated secondary antibodies.
• Validate the antibody in relevant positive and negative control samples, such as irradiated versus untreated cells.
• Titrate the antibody to determine optimal staining concentration for your specific experimental setup.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive IVD raw-material provider, offering validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents. For DNA damage research and diagnostic applications, CamelBio can support the sourcing of phospho-specific H2AX antibodies and related reagents to accelerate your assay development from concept to clinic.