PE Rabbit anti-Human NG2 mAb for Flow Cytometry - Q6UVK1

Core Product Specifications and Parameters

Biological Background: NG2 Function and Localization

• Protein identity: Chondroitin sulfate proteoglycan 4 (CSPG4), also known as NG2, MCSP (melanoma chondroitin sulfate proteoglycan), or HMW-MAA (high molecular weight-melanoma associated antigen).
• Core function: Involved in cell proliferation and migration, stimulating endothelial cell motility during microvascular morphogenesis.
• Neuronal modulation: May inhibit neurite outgrowth and induce growth cone collapse during axon regeneration.
• Extracellular interactions: Serves as a cell surface receptor for collagen alpha 2(VI), binds growth factors, and modulates extracellular matrix protease activity, thereby regulating MPP16-dependent degradation of type I collagen and enhancing plasminogen activation.
• Signal transduction: Functions as a signal transducer via its cytoplasmic C-terminus, activating pathways such as Rho GTPase (promoting retraction fiber formation), FAK, and ERK1/ERK2, as well as integrin-mediated adhesion through CDC42, ACK1, and BCAR1.
• Subcellular localization: Localizes to the cell membrane, apical cell membrane, lamellipodium membrane, and cell surface.
• Tissue specificity: Detected in fibroblasts, placenta, and malignant melanoma cells. Related references: PMID:36213313, PMID:32337544
• Key characteristics: Associated with angiogenesis, tissue remodeling, and differentiation; contains disulfide bonds, multiple glycosylation sites, and a transmembrane domain.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1583–2224 of the extracellular domain, enabling recognition of native NG2 on the cell surface. Consider validating specificity with appropriate NG2-overexpressing or knockout cell lines.
• For flow cytometry, titrate the antibody to determine the optimal concentration for your cell type, and include proper isotype controls, viability dyes, and Fc receptor blocking reagents to minimize nonspecific binding.
• NG2 is expressed in melanoma and some vascular cells; use positive (e.g., A375 melanoma cells) and negative (e.g., NG2-low lymphocytes) controls to establish gating and background levels.
• Fixation and permeabilization may affect epitope accessibility; if intracellular staining is required, test compatibility with your protocol.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to a comprehensive portfolio of IVD raw materials, technical services, and expert consulting—supporting projects from concept to clinic. This anti-NG2 monoclonal antibody is relevant for oncology and angiogenesis studies, and we can further supply validated antibody pairs, bulk ancillary reagents, and assist with sourcing rare target raw materials to streamline your diagnostic development or research workflows.