PE Rabbit anti-Human MRC2 Monoclonal Antibody for Flow Cytometry - Q9UBG0

Core Product Specifications and Parameters

Biological Background: MRC2 Function and Localization

• MRC2, also designated CD280, UPARAP, CLEC13E, and ENDO180, is a C-type mannose receptor 2 encoded by the MRC2 gene.
• It operates as a calcium-dependent endocytic lectin receptor that internalizes glycosylated ligands from the extracellular space via clathrin-mediated endocytosis, releasing them in endosomal compartments.
• The receptor participates in the plasminogen activation system, regulating the extracellular levels of PLAUR/PLAU and thereby modulating protease activity at the cell surface.
• MRC2 contributes to the uptake, remodeling, and degradation of extracellular collagen matrices, likely in cooperation with matrix metalloproteinases (MMPs).
• It may play a role in cancer progression and chronic tissue destructive diseases by influencing collagen turnover.
• The protein is a single-pass type I transmembrane receptor localized to the cell membrane.
• Its expression is ubiquitous but low in tissues including brain, placenta, lung, kidney, pancreas, spleen, thymus, and colon; it is highly expressed in fetal lung and kidney and found in endothelial cells, fibroblasts, and macrophages.
• Post-translational modifications include glycosylation, disulfide bond formation, phosphorylation, and ubiquitination (isopeptide bond).

Experimental Guidance and Technical Tips

• As a PE-conjugated antibody intended for direct flow cytometry, always include a matched isotype control to assess nonspecific staining.
• To determine the optimal concentration, perform a titration (e.g., 0.1–1 μg per 1×10⁶ cells) tested on a relevant human cell line known to express MRC2.
• MRC2 is a cell-surface receptor; maintain cell viability and validate any fixation/permeabilization protocol as it may affect epitope accessibility.
• For multicolor panels, confirm that the PE emission does not cause compensation issues with other fluorochromes in your instrument configuration.
• As a positive control, consider human fibroblasts, macrophages, or cancer cell lines; a negative control (e.g., a cell type with no detectable MRC2) helps set gating thresholds.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research institutes by offering a comprehensive range of IVD raw materials. This PE Rabbit anti-Human MRC2 monoclonal antibody is one example of our ready-to-use, application-optimized reagents for flow cytometry. We also provide antibody pair development, bulk ancillary reagents, and sourcing of rare target raw materials to accelerate your projects from concept to clinic.