PE Rabbit anti-Human mGluR2 Monoclonal Antibody for FC - Q14416

Core Product Specifications and Parameters

Biological Background: mGluR2 Function and Localization

• Metabotropic glutamate receptor 2 (mGluR2) is a dimeric G protein-coupled receptor (GPCR) activated by the excitatory neurotransmitter L-glutamate, critically modulating synaptic transmission and neuronal excitability (PubMed:37286794). Related references: PMID:37286794
• Upon activation, mGluR2 inhibits presynaptic calcium channels, reducing further glutamate release and dampening excitatory signaling.
• mGluR2 belongs to group II of the metabotropic glutamate receptor family, which comprises eight subtypes divided into three groups based on sequence homology and signaling mechanisms.
• The receptor is primarily localized at the cell membrane, enriched at synapses and on dendrites, where it serves as a presynaptic autoreceptor.
• Post-translational modifications include N-linked glycosylation and disulfide bond formation, typical of class C GPCRs, contributing to its molecular weight of approximately 96 kDa.
• Beyond neurotransmission, mGluR2 acts as a host entry factor for several viruses, including influenza, rabies, and SARS-CoV-2, hijacking its endocytosis pathway for cell entry.
• mGluR2 is widely expressed in the adult brain, with robust detection in the cerebral cortex, and is also present in fetal brain tissue.

Experimental Guidance and Technical Tips

• This PE-conjugated antibody enables direct flow cytometric detection of native cell surface mGluR2 without the need for secondary reagents.
• The immunogen targets amino acids 22-498, a large extracellular region; thus, the antibody likely recognizes an extracellular epitope accessible on live cells.
• Validate the antibody on human cell lines expressing mGluR2, such as neuronal or brain-derived lines, and include appropriate isotype controls.
• For surface staining, use fresh or lightly fixed cells; permeabilization is typically unnecessary but may be required for intracellular or total mGluR2 detection.
• Given mGluR2’s role in viral entry, this antibody may be applied in infection assays to monitor receptor expression, but further validation is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your dedicated one-stop sourcing partner for IVD raw materials, offering a comprehensive portfolio that spans from validated antibody pairs and optimized monoclonal/polyclonal antibodies to bulk ancillary reagents and rare target sourcing. This anti-mGluR2 antibody supports research into neurology and infectious disease mechanisms, and CamelBio’s integrated supply chain and technical consulting can accelerate your project from concept to clinical application.