PE Rabbit anti-Human MERTK Monoclonal Antibody for Flow Cytometry - Q12866

Core Product Specifications and Parameters

Biological Background: MERTK Function and Localization

• MERTK is a receptor tyrosine kinase that transduces extracellular signals by binding ligands such as LGALS3, TUB, TULP1, and GAS6.
• Upon ligand binding, MERTK autophosphorylates and interacts with GRB2 or PLCG2, leading to phosphorylation of downstream effectors including MAPK1, MAPK2, FAK/PTK2, or RAC1, thereby regulating processes like cytoskeleton reorganization and engulfment. Related references: PMID:32640697
• It plays a critical role in macrophage-mediated clearance of apoptotic cells (efferocytosis) and platelet aggregation.
• In the retinal pigment epithelium (RPE), MERTK functions as a regulator of phagocytosis of rod outer segment fragments.
• MERTK inhibits Toll-like receptor (TLR)-mediated innate immune responses by activating STAT1, which induces production of SOCS1 and SOCS3.
• The protein is localized at the cell membrane and contains characteristic domains such as immunoglobulin domains, a kinase domain, and is subject to glycosylation and phosphorylation.
• Tissue expression: highly expressed in testis, ovary, prostate, lung, and kidney; lower in spleen, small intestine, colon, and liver; not detected in normal B- and T-lymphocytes but present in many neoplastic B- and T-cell lines.

Experimental Guidance and Technical Tips

• As this antibody is PE-conjugated, it is suitable for direct flow cytometry. Validate the antibody in relevant human cell lines or primary cells expressing MERTK.
• Consider titrating the antibody to optimize signal-to-noise ratio for your specific cell type and experimental conditions.
• MERTK expression may be modulated in certain physiological or pathological states; confirm target expression prior to staining.
• The immunogen covers amino acids 26-499 within the extracellular region, so the antibody may recognize native surface MERTK; however, always confirm reactivity in your assay system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive one-stop sourcing solution for diagnostic manufacturers and research institutes, offering validated monoclonal antibodies like this anti-MERTK antibody. Our portfolio includes bulk ancillary reagents and customized raw material sourcing for oncology and immunology research. Partner with us to streamline your supply chain from concept to clinic.