PE Rabbit anti-Human LIFR/CD118 Monoclonal Antibody for FC - P42702

Core Product Specifications and Parameters

Biological Background: LIFR Function and Localization

• LIFR functions as a membrane-bound signal-transducing subunit, forming complexes with IL6ST/gp130 or with CNTFR and IL6ST/gp130 to mediate signaling for cytokines such as LIF, OSM, CNTF, CTF1, and CLCF1.
  Related references: PMID:11285233 PMID:11294841 PMID:1542794
• Ligand binding induces heterodimerization of IL6ST/gp130 and LIFR, activating JAK kinases that phosphorylate the receptor and downstream STAT transcription factors.
  Related references: PMID:11285233 PMID:11294841 PMID:9030543
• It engages site 3 of cytokine ligands, as shown in structural studies.
  Related references: PMID:36930708
• A soluble isoform of LIFR can inhibit the biological activity of LIF by blocking its binding to target cells.
• The receptor is a glycoprotein with multiple disulfide bonds and is subject to phosphorylation.
• Subcellularly, LIFR localizes to the cell membrane, with secreted variants likely arising from alternative splicing.
• Mutations in LIFR are associated with diseases such as Stüve-Wiedemann syndrome (SWS, SJS2, STWS), underscoring its physiological importance.

Experimental Guidance and Technical Tips

• This antibody is directly conjugated to PE, enabling direct single-step staining for flow cytometry without the need for a secondary antibody.
• The immunogen spans the extracellular domain (amino acids 45–833), which may recognize both membrane-bound and soluble LIFR; consider including an appropriate isotype control and Fc block in your FC panel.
• Titrate the antibody to determine the optimal concentration for your cell preparation, as signal intensity can vary with antigen density and cell type.
• Validate antibody performance in known LIFR-expressing human cell lines (e.g., leukocytes, certain tumor lines) and consider using physiological stimuli (e.g., LIF, OSM) to modulate receptor expression.
• For intracellular detection, include a fixation/permeabilization step; note that PE fluorescence may be sensitive to certain permeabilization buffers.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories by providing reliable access to high-quality IVD raw materials, including this PE-conjugated anti-LIFR monoclonal antibody for flow cytometry applications. Beyond individual antibodies, we offer validated antibody pairs, bulk ancillary reagents, and custom sourcing services for rare targets, helping you streamline assay development from concept to clinic. Whether you need a complete cytokine receptor signaling panel or specialized conjugate formats, our one-stop platform bridges the gap between research and commercialization.