PE Rabbit anti-Human LGR5 (GPR49) Monoclonal Antibody for Flow Cytometry - O75473

Core Product Specifications and Parameters

Biological Background: LGR5 Function and Localization

• LGR5, also known as GPR49, GPR67, and HG38, is a leucine-rich repeat-containing G protein-coupled receptor that functions as a receptor for R-spondins.
• It potentiates the canonical Wnt signaling pathway by associating with phosphorylated LRP6 and frizzled receptors upon R-spondin binding, increasing target gene expression.
• Unlike classical GPCRs, LGR5 does not activate heterotrimeric G proteins to transduce signals.
• LGR5 is a well-established stem cell marker for intestinal epithelium and hair follicle, playing a crucial role in the development and maintenance of adult intestinal stem cells during postembryonic development.
• Subcellularly, LGR5 localizes to the cell membrane, Golgi apparatus, and trans-Golgi network membrane.
• It is expressed in skeletal muscle, placenta, spinal cord, and brain regions, and specifically at the base of crypts in colonic and small intestine mucosa stem cells; overexpression is observed in ovarian, colon, and liver cancers.
• The protein is subject to alternative splicing and contains key structural features such as disulfide bonds, a glycoprotein nature, leucine-rich repeats, and seven transmembrane helices.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a C-terminal intracellular region (amino acids 480-580), potentially suitable for detecting total LGR5 expression; verify epitope accessibility in your flow cytometry protocol.
• For flow cytometry, ensure appropriate cell permeabilization if targeting intracellular epitopes, or use surface staining protocols if the epitope is surface-exposed.
• Validate antibody performance in relevant cell types known to express LGR5, such as intestinal organoids, colorectal cancer cell lines, or hair follicle cells, and include positive and negative controls.
• Given low LGR5 expression in some stem cell populations, consider signal amplification strategies or the use of recombinant protein controls to optimize detection.

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