PE Rabbit anti-Human Lambda Light Chain Monoclonal Antibody for Flow Cytometry - P0CG04

Core Product Specifications and Parameters

Biological Background: Immunoglobulin Lambda Constant 1 Function and Localization

• This product targets the constant region of human immunoglobulin lambda light chains (Ig lambda chain C region, also known as MGC or Ig lambda-1 chain C region), which are essential components of antibody molecules.
• Secreted immunoglobulins, through their constant regions, mediate effector functions such as complement activation and Fc receptor engagement, thereby eliminating antigens. Related references: PMID:20176268, PMID:22158414
• Membrane-bound immunoglobulins act as B cell receptors; antigen binding triggers B cell clonal expansion and differentiation into antibody-secreting plasma cells. Related references: PMID:20176268
• Antigen binding specificity arises from the variable domains assembled via V(D)J recombination. Somatic hypermutation after antigen exposure further refines antibody affinity. Related references: PMID:17576170, PMID:20176268
• The protein is found both secreted into extracellular fluids and anchored on the plasma membrane of B lymphocytes, consistent with dual roles in humoral immunity.
• Structurally, the constant domain contains conserved intrachain disulfide bonds that maintain the immunoglobulin fold. Its detection as Bence-Jones protein in urine is associated with plasma cell dyscrasias.
• The human genome encodes multiple functional IGLC genes; IGLC1 (this target) is one of several lambda constant region genes.

Experimental Guidance and Technical Tips

• The immunogen covers the full-length constant domain (amino acids 1-106), suggesting the antibody recognizes all human lambda light chain isotypes. Validate specificity against kappa light chain-expressing cells in your flow cytometry panel.
• As a PE-conjugated monoclonal antibody, it is suitable for direct immunofluorescence staining. Titrate the antibody to determine the optimal concentration for your cell type and protocol.
• For surface immunoglobulin staining, use standard cell surface staining procedures. For intracellular light chain detection, incorporate a permeabilization step after fixation.
• Include appropriate isotype control antibodies and compensation controls in multicolor flow cytometry experiments to ensure accurate data interpretation.
• The predicted molecular weight of the constant domain alone is ~11 kDa; however, under non-reducing conditions, light chains may appear at ~25 kDa due to disulfide bonding and association with heavy chains.

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CamelBio serves as your comprehensive one-stop sourcing bridge for IVD raw materials, providing diagnostic manufacturers and research laboratories with reliable access to antibody products like this anti-lambda light chain monoclonal antibody. Our portfolio includes validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare-target sourcing capabilities to accelerate your assay development from concept to clinic. Partner with us for your immunology and hematology research reagent needs.