PE Rabbit anti-Human KIR3DL1/CD158e Monoclonal Antibody for FC - P43629

Core Product Specifications and Parameters

Biological Background: KIR3DL1/CD158e Function and Localization

• KIR3DL1, also designated CD158e, NKAT3, NKB1, and p70 natural killer receptor, is an inhibitory receptor expressed on NK cells.
• It specifically recognizes HLA-Bw4 allotypes and inhibits NK cell cytotoxicity, contributing to immune self-tolerance.
• This receptor belongs to the killer cell immunoglobulin-like receptor (KIR) family, which includes both inhibitory and activating members.
• KIR3DL1 is a type I transmembrane glycoprotein located on the cell surface.
• The extracellular region comprises immunoglobulin-like domains, and the protein undergoes glycosylation and contains disulfide bonds.
• Alternative splicing produces multiple isoforms, which may modulate receptor function.

Experimental Guidance and Technical Tips

• This PE-conjugated antibody is designed for direct flow cytometric detection of KIR3DL1 on NK cells without the need for secondary staining.
• Consider using appropriate isotype controls and live/dead cell discrimination to ensure accurate gating.
• Validate antibody staining with known KIR3DL1-positive and negative cell lines or primary human NK cells.
• The immunogen corresponds to the extracellular domain (aa 22–340), so permeabilization is not required for cell surface labeling.
• For multi-color flow cytometry panels, carefully optimize compensation settings due to the bright PE conjugate.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with comprehensive access to high-quality IVD raw materials. Our KIR3DL1 antibody products support NK cell immunophenotyping and immune-oncology research, backed by a full portfolio of antibody pairs, recombinant proteins, and ancillary reagents. From concept to clinic, CamelBio streamlines your raw-material sourcing needs.