PE Rabbit anti-Human KIR2DL4/CD158d Monoclonal Antibody for Flow Cytometry - Q99706

Core Product Specifications and Parameters

Biological Background: KIR2DL4 Function and Localization

• KIR2DL4 is a killer cell immunoglobulin-like receptor that functions as a receptor for non-classical MHC class Ib molecule HLA-G, specifically recognizing peptide-bound HLA-G-B2M complexes.
• In decidual NK cells, KIR2DL4 binding to peptide-bound HLA-G-B2M triggers a senescence-associated secretory phenotype, promoting vascular remodeling and fetal growth during early pregnancy.
  Related references: PMID:16366734, PMID:23184984, PMID:29262349
• May help balance tolerance and antiviral immunity at the maternal-fetal interface by modulating the effector functions of NK cells, CD8+ T cells, and B cells.
  Related references: PMID:10190900, PMID:16366734
• Upon ligand engagement, KIR2DL4 signals from endosomal compartments, activating PRKDC-XRCC5 and AKT1 to drive NF-kappa-B-dependent pro-inflammatory responses.
  Related references: PMID:20179272
• Localizes to the cell membrane and early endosome membrane.
• Expressed in decidual NK cells, innate lymphoid cells type 1 (ILC1), and a subset of peripheral NK cells.
  Related references: PMID:29262349, PMID:19304799
• Structurally, it is a member of the immunoglobulin superfamily, containing Ig-like domains, disulfide bonds, and glycosylation sites; multiple isoforms are generated through alternative splicing.

Experimental Guidance and Technical Tips

• As a PE-conjugated antibody, this reagent enables direct detection in flow cytometry; include appropriate compensation controls in multicolor panels.
• The immunogen (aa 24-242) covers the extracellular domain, suggesting the antibody likely targets an epitope accessible in live-cell staining; consider evaluating its blocking potential in functional assays.
• Validate staining specificity in your cell system using isotype controls and Fc receptor blocking, particularly when working with NK cells or ILC1s that may express Fc receptors.
• Titrate the antibody to determine the optimal concentration for your experimental conditions.
• Due to alternative splicing, KIR2DL4 expression levels and isoforms may vary among cell subsets; verify expression in your target population.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research institutes with a comprehensive portfolio of IVD raw materials and technical services, from early-stage development to clinical application. This anti-KIR2DL4 antibody serves as a key tool for investigating NK cell biology, immune tolerance, and reproductive immunology. Complement your studies with CamelBio’s validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and expert support for rare target material sourcing.