PE Rabbit anti-Human Integrin alpha 3/CD49c Monoclonal Antibody for Flow Cytometry - P26006

Core Product Specifications and Parameters

Biological Background: Integrin alpha-3 Function and Localization

• Integrin alpha-3 (UniProt: P26006, also known as CD49c, VLA-3 alpha chain, GAPB3) is a transmembrane glycoprotein that heterodimerizes with integrin beta-1 to form the alpha-3/beta-1 receptor. The alpha subunit contains a large extracellular domain with seven FG-GAP repeats, a transmembrane helix, and a short cytoplasmic tail.
• ITGA3/ITGB1 serves as a versatile adhesion receptor for multiple extracellular matrix ligands, including fibronectin, laminin, collagen, epiligrin, thrombospondin, and CSPG4, mediating cell–matrix interactions critical for tissue organization.
• At invadopodia plasma membranes, integrin alpha-3/beta-1 provides a collagen-dependent docking site for fibroblast activation protein (FAP/seprase), facilitating adhesion, invadopodia formation, and matrix degradation to promote cell invasion.
• In endothelial cells, alpha-3/beta-1 may cooperate with LGALS3 to mediate CSPG4-triggered cell migration, linking the receptor to angiogenic and wound-healing processes.
• (Microbial infection) ITGA3:ITGB1 may act as a cellular receptor for Rhizopus delemar CotH7 on alveolar epithelial cells, representing a potential early step in the pathogenesis of pulmonary mucormycosis.
• The protein localizes predominantly to the cell membrane and is enriched in cell projections such as invadopodia and filopodia, consistent with its roles in adhesion and motility.
• Two major isoforms are produced by alternative splicing: isoform 1 is widely expressed, while isoform 2 is detected primarily in brain and heart. In brain, both isoforms are restricted to vascular smooth muscle cells; in heart, isoform 1 is found on vascular smooth muscle cells and isoform 2 on endothelial vein cells.
• Integrin alpha-3 undergoes several post-translational modifications, including N-glycosylation, palmitoylation, phosphorylation, and cleavage on basic residues, all of which may regulate receptor trafficking, affinity, and signaling. Mutations in ITGA3 are linked to junctional epidermolysis bullosa with interstitial lung disease and nephrotic syndrome.

Experimental Guidance and Technical Tips

• As a PE-conjugated monoclonal antibody, this reagent is suitable for direct immunofluorescence flow cytometry on live or fixed human cells. Validate optimal working concentration by titration (e.g., 0.1–1 µg per million cells) in the relevant sample system.
• The immunogen encompasses amino acids 466–620 of human Integrin alpha-3 (within the extracellular domain), a region conserved in both isoforms. Confirm reactivity with isoform 2-expressing primary cells or transfected lines if required.
• For surface staining, harvest cells without trypsin if possible, as trypsin may cleave surface integrins; consider using non-enzymatic dissociation reagents. Include appropriate isotype and fluorescence-minus-one controls.
• Given the receptor’s involvement in cell adhesion, activation-dependent conformational changes might affect epitope accessibility; test under resting and stimulated conditions if applicable.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic and life science organizations with comprehensive access to IVD raw materials, serving as a single sourcing bridge from target identification to clinical application. For Integrin alpha-3/CD49c—a key adhesion receptor implicated in tumor invasion, wound healing, and rare diseases—we provide validated monoclonal antibodies (including PE conjugates), matching antibody pairs, recombinant proteins, and ancillary reagents to accelerate assay development. Whatever your project’s scale or specificity, rely on CamelBio for streamlined procurement of high-quality raw materials and tailored technical support.