PE Rabbit anti-Human ILT5/CD85a Monoclonal Antibody for FC - O75022

Core Product Specifications and Parameters

Biological Background: ILT5/CD85a Function and Localization

• ILT5/CD85a is a leukocyte immunoglobulin-like receptor subfamily B member 3 (LILRB3), also known as CD85a, immunoglobulin-like transcript 5 (ILT-5), and monocyte inhibitory receptor HL9.
• It may function as a receptor for class I MHC antigens and, upon coligation with immune receptors such as FCGR2B and the B-cell receptor, down-regulates antigen-induced B-cell activation by recruiting phosphatases to its immunoreceptor tyrosine-based inhibitory motifs (ITIMs).
• The protein is localized to the cell membrane, consistent with its role as a transmembrane inhibitory receptor.
• Tissue expression is detected in monocytes and B-cells, indicating a role in both innate and adaptive immune regulation.
• Structural features include immunoglobulin domains, a transmembrane helix, and a signal peptide; the protein undergoes alternative splicing, glycosylation, and phosphorylation.
• Key ontological classifications include adaptive immunity, disulfide bond, glycoprotein, phosphoprotein, receptor, repeat, and transmembrane.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (amino acids 24-418) of human ILT5/CD85a, which may be suitable for detecting native surface receptor expression.
• For flow cytometry (FC), consider titrating the antibody using known positive controls (e.g., human monocytes or B-cell lines) and negative controls to establish optimal working concentration.
• Use appropriate isotype controls and Fc receptor blocking reagents to minimize non-specific binding.
• Since the antibody is PE-conjugated, include compensation controls when used in multicolor panels.
• Verify expression of the target in your sample system, as ILT5/CD85a is primarily detected in monocytes and B-cells.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines your IVD raw material procurement by providing one-stop access to validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target sourcing for immune checkpoint and inhibitory receptor research. For ILT5/CD85a-related projects, we can supply complementary reagents such as detection antibodies, recombinant proteins, and isotype controls, tailored to diagnostic development and research needs. Let CamelBio be your trusted partner in bridging concept to clinic with reliable, quality-controlled raw materials.