PE Rabbit anti-Human IL9 mAb - P15248

Core Product Specifications and Parameters

Biological Background: IL-9 Function and Localization

• Interleukin-9 (IL-9), also known as Cytokine P40 and T-cell growth factor P40, is a multifunctional cytokine encoded by the IL9 gene in humans.
• It is primarily secreted by T-helper 2 lymphocytes, mast cells, and NKT cells.
• IL-9 plays a critical role in immune responses against parasites and influences intestinal epithelial permeability and adaptive immunity. Related references: PMID:29742432
• It promotes the differentiation of IL-17 producing helper T-cells (TH17) and the proliferation and differentiation of mast cells.
• Mechanistically, IL-9 exerts its effects through a receptor complex composed of IL9R and IL2RG, leading to activation of JAK1 and JAK3 kinases and downstream STAT1, STAT3, and STAT5 signaling.
• The protein is secreted and undergoes post-translational modifications including glycosylation and formation of pyrrolidone carboxylic acid.
• According to UniProt, it is classified under keywords such as cytokine, glycoprotein, growth factor, and secreted.

Experimental Guidance and Technical Tips

• This PE-conjugated rabbit monoclonal antibody is designed for intracellular flow cytometry (FC intra) detection of human IL-9. It may require a permeabilization step optimized for cytokines.
• Consider titrating the antibody to determine the optimal working concentration for your cell type and experimental setup, and include appropriate isotype and fluorescence minus one controls.
• Stimulation of human immune cells (e.g., with PMA/ionomycin) may be necessary to induce IL-9 expression before staining; validate the stimulation protocol in your relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a one-stop sourcing platform for IVD raw materials, supporting diagnostic manufacturers, laboratories, and research institutes from concept to clinic. This anti-IL9 antibody targets a key cytokine in immunology research, and we can supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare-target raw materials to streamline your procurement needs. Contact us to leverage our integrated supply capabilities.