PE Rabbit anti-Human IL18R1/IL-18 R alpha Monoclonal Antibody for FC - Q13478

Core Product Specifications and Parameters

Biological Background: IL18R1 Function and Localization

• IL18R1 is the ligand-binding subunit of the IL-18 receptor complex, specifically recognizing the pro-inflammatory cytokine IL-18, but not IL-1A or IL-1B. Related references: PMID:14528293 PMID:25261253 PMID:25500532
• It plays a critical role in IL-18-mediated interferon-gamma (IFNG) production from T-helper 1 (Th1) cells, enhancing Th1-type immune responses. Related references: PMID:10653850
• IL18R1 belongs to the interleukin-1 receptor family and contains immunoglobulin-like domains in its extracellular region.
• The receptor is a membrane protein with a single transmembrane helix, consistent with its role in signal transduction.
• Post-translational modifications include glycosylation and disulfide bond formation, which are important for structural stability and function.
• Highly expressed in leukocytes, spleen, and lung; lower expression in liver, small intestine, colon, prostate, thymus, placenta, and heart. It is specifically coexpressed with IL18R1 in Th1 cells. Related references: PMID:10653850 PMID:10925275 PMID:11046021
• Keywords from UniProt include inflammatory response, glycoprotein, and receptor, highlighting its role in immune signaling pathways.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 22-329, which covers the extracellular region; thus the antibody likely recognizes the ectodomain, suitable for surface staining in live cells.
• Because this antibody is directly conjugated to PE and intended for flow cytometry (FC), titrate the antibody to determine optimal staining concentration using relevant cell lines or primary cells.
• Since IL18R1 is a membrane protein, avoid permeabilization steps unless intracellular staining is desired; use appropriate isotype controls for accurate gating.
• Consider co-staining with other Th1 markers to evaluate IL18R1 expression in T-cell subsets.
• For functional blocking studies, note that binding of this antibody to its epitope has not been validated to neutralize IL-18 signaling; further optimization may be required.
• Validate performance in the specific sample system of interest, as expression levels may vary across cell types and activation states.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents. For targets like IL18R1 involved in immunological and inflammatory pathways, we support the procurement of specialized research materials to accelerate assay development. Our portfolio addresses the needs of diagnostic manufacturers and research laboratories from concept to clinical application.