PE Rabbit anti-Human IL-5 Monoclonal Antibody for Flow Cytometry - P05113

Core Product Specifications and Parameters

Biological Background: IL-5 Function and Localization

• IL-5 is a homodimeric cytokine expressed predominantly by T-lymphocytes and NK cells, playing a critical role in eosinophil survival, differentiation, and chemotaxis. Related references: PMID:2653458 / PMID:9010276
• Additionally, IL-5 acts on activated and resting B-cells to promote immunoglobulin production, growth, and differentiation (By similarity).
• IL-5 signals through a receptor complex composed of IL5RA and the common beta subunit CSF2RB; binding activates kinases (LYN, SYK, JAK2) and propagates signals via the RAS-MAPK and JAK-STAT5 pathways. Related references: PMID:1495999 / PMID:22528658 / PMID:7613138
• IL-5 is a secreted protein, consistent with its role as a cytokine.
• Tissue expression includes peripheral blood mononuclear cells.
• Post-translational modifications include glycosylation and disulfide bond formation; alternative splicing generates multiple isoforms.

Experimental Guidance and Technical Tips

• The immunogen, comprising amino acids 20-134 of human IL-5, corresponds to the mature secreted form; consider this design when planning detection of endogenous or recombinant IL-5.
• For intracellular flow cytometry (FC (intra)), optimize fixation and permeabilization protocols for your specific cell type, and include appropriate isotype controls.
• Validate performance in relevant human sample systems; cross-reactivity with other species is not indicated in the provided specifications.
• If detecting low-abundance endogenous IL-5, consider cell stimulation to enhance signal; titrate the antibody to reduce background.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio acts as a comprehensive one-stop sourcing partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing. With this PE Rabbit anti-Human IL-5 mAb, researchers in immunology and cytokine biology can access a key tool for eosinophil and B-cell studies. CamelBio streamlines procurement from concept to clinic, ensuring reliable supply for diagnostic development.