PE Rabbit anti-Human IL-4 mAb - P05112

Core Product Specifications and Parameters

Biological Background: IL-4 Function and Localization

• IL-4, also known as B-cell stimulatory factor 1 (BSF-1), is a cytokine encoded by the IL4 gene, with alternative splicing producing multiple isoforms.
• Secreted primarily by mast cells, T-cells, eosinophils, and basophils.
• It induces expression of class II MHC molecules on resting B-cells, enhances secretion and surface expression of IgE and IgG1, and regulates CD23 expression on lymphocytes and monocytes.
  Related references: PMID:1993171 PMID:2521231
• IL-4 signals through two receptor complexes: type 1 (IL4R/IL2RG) on hematopoietic cells and type 2 (IL4R/IL13RA1) on nonhematopoietic cells, leading to JAK/STAT6 activation.
  Related references: PMID:10219247 PMID:11526337 PMID:18243101 PMID:7721895
• Additionally, IL-4 positively regulates IL31RA in macrophages, stimulates autophagy in dendritic cells via mTORC1 and RUFY4, and plays a role in brain functions such as memory and learning (By similarity).
• It is a glycoprotein containing disulfide bonds and is secreted into the extracellular space.

Experimental Guidance and Technical Tips

• For intracellular flow cytometry (FC), optimize fixation and permeabilization protocols to ensure adequate detection of the target.
• Perform antibody titration using relevant cell types and activation conditions to determine the optimal staining concentration.
• As a PE-conjugated antibody, plan compensation controls appropriately for multicolor flow cytometry panels.
• Given the immunogen corresponds to the mature IL-4 sequence (aa 25–153), it is expected to recognize both nascent intracellular and secreted forms after permeabilization.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw-material procurement by providing a single access point for validated antibodies, bulk ancillary reagents, and rare-target sourcing. For cytokine-focused diagnostic development or immune monitoring panels, our supply solutions—including anti‑IL‑4 reagents—support consistent assay performance from concept to clinic. Reach out to explore how we can accelerate your workflow.