PE Rabbit anti-Human IL-2 Monoclonal Antibody for Flow Cytometry (Intracellular) - P60568

Core Product Specifications and Parameters

Biological Background: IL-2 (Interleukin-2) Function and Localization

• Interleukin-2 (IL-2), also known as T-cell growth factor (TCGF), is a secreted cytokine of the interleukin family.
• IL-2 is primarily produced by activated CD4-positive helper T-cells, with minor production by activated CD8-positive T-cells and natural killer (NK) cells. Related references: PMID:6438535
• It binds to a receptor complex composed of either the high-affinity trimeric IL-2R (IL2RA/CD25, IL2RB/CD122 and IL2RG/CD132) or the low-affinity dimeric IL-2R (IL2RB and IL2RG). Related references: PMID:16293754, PMID:16477002
• Receptor engagement triggers downstream signaling via phosphorylation of JAK1 and JAK3, leading to STAT5 activation and the stimulation of PI3K and MAPK pathways. Related references: PMID:7973659, PMID:8580378, PMID:25142963
• IL-2 functions as a T-cell growth factor and enhances the cytolytic activity of NK cells. Related references: PMID:6608729
• It promotes strong proliferation of activated B-cells and subsequent immunoglobulin production. Related references: PMID:6438535
• IL-2 plays a pivotal role in regulating the adaptive immune system by controlling the survival and proliferation of regulatory T-cells, and it participates in the differentiation and homeostasis of effector T-cell subsets, including Th1, Th2, Th17, and memory CD8-positive T-cells.
• The mature protein is a glycosylated, disulfide-bonded monomer that is secreted from cells.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the mature IL-2 sequence (amino acids 21–153) without the signal peptide, ensuring specificity for the processed, secreted form of human IL-2.
• For intracellular flow cytometry, optimize fixation and permeabilization protocols; consider using PMA and ionomycin stimulation of T-cells to enhance IL-2 production prior to staining.
• The PE conjugation allows direct detection without secondary reagents; titrate the antibody to determine the optimal concentration for your sample system.
• Cross-reactivity has only been confirmed for human IL-2; if using other species, validate specificity experimentally.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with comprehensive access to IVD raw materials, from validated antibody pairs to bulk ancillary reagents. This PE Rabbit anti-Human IL-2 monoclonal antibody supports immune monitoring and therapeutic development in flow cytometry applications. For custom rare-target sourcing and expert technical consultation, contact the CamelBio team.