PE Rabbit anti-Human IL-17RA/CD217 mAb for FC - Q96F46

Core Product Specifications and Parameters

Biological Background: Interleukin-17 receptor A Function and Localization

• Functions as a receptor for IL-17A and IL-17F, binding IL-17A with higher affinity, and mediates innate and adaptive immune responses involved in host defense and tissue integrity. Related references: PMID:17911633 PMID:9367539 PMID:19838198
• Forms a heterodimeric complex with IL-17RC to bind IL-17A, IL-17F homodimers and IL-17A/IL-17F heterodimers. Related references: PMID:16785495 PMID:18684971
• Cytokine binding triggers TRAF3IP2 adapter-dependent TRAF6-mediated activation of NF-kappa-B and MAP kinase pathways, driving transcription of pro-inflammatory cytokines, chemokines, antimicrobial peptides, and MMPs. Related references: PMID:16785495 PMID:17911633 PMID:18684971
• Also functions as a receptor for IL-17C when forming a heterodimeric complex with IL-17RE. Related references: PMID:21993848
• Contributes to antimicrobial host defense by promoting neutrophil activation and recruitment, and stimulating antimicrobial beta-defensins production from mucosal epithelia.
• Plays a role in antiviral host defense, including T cell cytotoxicity against West Nile virus and B-1a B cell-mediated influenza virus clearance.
• Predominantly localized to the cell membrane and can be secreted; post-translationally modified by glycosylation and phosphorylation.
• Widely expressed across tissues.

Experimental Guidance and Technical Tips

• The immunogen spans the extracellular domain (aa 33-320), making the antibody well-suited for detecting cell surface IL-17RA by flow cytometry.
• For FC analysis, use standard surface staining protocols; ensure appropriate PE compensation controls are included to correct spectral overlap.
• Validate the antibody on IL-17RA-expressing immune cell lines (e.g., Th17 cells, fibroblasts, epithelial cells) and include an isotype control to confirm specificity.
• As IL-17RA can be shed from the membrane, consider using secretion inhibitors or staining under non-permeabilizing conditions for surface-only detection.
• When multiplexing with other markers, verify fluorophore compatibility and optimal antibody concentrations to avoid steric hindrance or saturation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a one-stop sourcing partner for IVD raw materials, supporting diagnostic manufacturers and research labs from early development to clinical application. This IL-17RA antibody targets a key immunology marker; we offer a comprehensive portfolio including validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets. Our integrated supply chain and technical expertise streamline assay development, ensuring reliable access to the critical materials you need.