PE Rabbit anti-Human IL-10RA/CD210 Monoclonal Antibody for Flow Cytometry - Q13651

Core Product Specifications and Parameters

Biological Background: IL-10RA/CD210 Function and Localization

• Interleukin-10 receptor subunit alpha (IL-10RA), also designated CD210, CDw210a, IL-10R1, or IL10R, is the ligand-binding subunit of the IL-10 receptor complex, a class II cytokine receptor.
• IL-10RA binds the anti-inflammatory cytokine IL-10 and, upon binding, induces a conformational change in IL-10RB, enabling the formation of a heterotetrameric receptor complex consisting of two IL-10RA and two IL-10RB subunits. Related references: PMID:16982608
• This assembly triggers the activation of the constitutively associated kinases JAK1 (via IL-10RA) and TYK2 (via IL-10RB), leading to phosphorylation of specific tyrosine residues in the intracellular domain of IL-10RA and subsequent recruitment and activation of STAT3. Related references: PMID:12133952
• Activated STAT3 homodimerizes and translocates to the nucleus to drive the expression of anti-inflammatory target genes, thereby limiting tissue damage during inflammatory responses.
• STAT3 signaling via IL-10RA also suppresses starvation-induced autophagy, highlighting its role in metabolic regulation. Related references: PMID:26962683
• IL-10RA is a transmembrane glycoprotein; it contains a signal peptide, a single transmembrane helix, and an intracellular domain with multiple phosphorylation and ubiquitination sites, as well as conserved disulfide bonds.
• The protein localizes primarily to the cell membrane and is also detected in the cytoplasm.
• Tissue expression is restricted to hematopoietic lineages, including B-cells, T-cells, NK cells, monocytes, and macrophages; it is absent from non-hematopoietic cells such as fibroblasts and endothelial cells.

Experimental Guidance and Technical Tips

• The antibody was generated against a recombinant fusion protein spanning the extracellular domain (amino acids 22-235) of human IL-10RA; it is predicted to recognize the native cell surface receptor.
• As a PE-conjugated monoclonal antibody, it is designed for direct flow cytometry; consider titrating the antibody to determine the optimal concentration for your specific cell system.
• Include appropriate isotype controls and Fc receptor blocking steps to minimize non-specific binding when analyzing primary hematopoietic cells.
• IL-10RA is constitutively expressed on B-cells, T-cells, NK cells, monocytes, and macrophages; therefore, human PBMCs or immune cell lines can serve as positive control samples.
• Validate surface staining by comparing with known expression profiles; note that IL-10RA is not expressed on non-hematopoietic cells like fibroblasts.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research labs with a one-stop sourcing platform for IVD raw materials, covering every stage from concept to clinic. Our portfolio includes validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents for targets such as IL-10RA/CD210, facilitating assays for anti-inflammatory signaling and immune profiling. Whether you need PE-conjugated detection reagents or rare receptor antibodies, CamelBio streamlines your procurement and accelerates development.