PE Rabbit anti-Human IgG (Fc) Monoclonal Antibody for Flow Cytometry - P01857

Core Product Specifications and Parameters

Biological Background: Immunoglobulin Heavy Constant Gamma 1 (IGHG1) Function and Localization

• IGHG1 encodes the constant region of the gamma 1 heavy chain; alternative splicing produces both membrane‑bound (B cell receptor) and secreted (antibody) isoforms.
• Membrane‑bound IgG1 serves as the antigen receptor on B lymphocytes; antigen binding triggers clonal expansion and differentiation into plasma cells.
  Related references: PMID:17576170 PMID:20176268 PMID:22158414
• Secreted IgG1 antibodies bind antigens with high affinity, achieved through V(D)J recombination, somatic hypermutation, and affinity maturation.
  Related references: PMID:17576170 PMID:20176268 PMID:22158414
• The Fc region of IgG1 interacts with Fcγ receptors on innate immune cells and with complement C1q to mediate effector functions such as antibody‑dependent cellular cytotoxicity (ADCC), phagocytosis, and complement‑dependent cytotoxicity (CDC).
• IgG1 is a glycoprotein containing intra‑ and inter‑chain disulfide bonds that stabilize the immunoglobulin fold.
• The protein is located at the cell membrane as part of the B cell receptor and is secreted as soluble antibody.
• Class‑switch recombination replaces the constant region of IgM/IgD with that of IgG1, enabling B cells to switch isotype and tailor effector functions.

Experimental Guidance and Technical Tips

• Titrate the antibody in the intended cell system to determine the optimal concentration for flow cytometry.
• Consider pre‑incubating cells with an Fc receptor blocking reagent to minimize nonspecific binding when working with Fc‑receptor‑expressing cells.
• Include appropriate compensation controls (single‑stain and fluorescence minus one) for PE channel to ensure accurate data analysis.
• Verify reactivity in human samples; cross‑reactivity with other species has not been determined. If using on non‑human primate cells, validate experimentally.
• Protect the antibody from prolonged light exposure to maintain PE fluorescence stability.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials, including this PE‑conjugated rabbit anti‑human IgG Fc monoclonal antibody for immunology and antibody‑detection applications. We provide validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and sourcing support for rare targets to streamline your diagnostic development from research to clinical use. Partner with CamelBio for reliable, one‑stop access to critical raw materials.