PE Rabbit anti-Human IgG (Fc) Monoclonal Antibody for Flow Cytometry - P01857

Core Product Specifications and Parameters

Biological Background: Immunoglobulin Heavy Constant Gamma 1 (IGHG1) Function and Localization

• IGHG1 encodes the constant region of the immunoglobulin gamma-1 heavy chain, a core component of IgG1 antibodies that mediates humoral immunity.
  Related references: PMID:17576170 PMID:20176268 PMID:22158414
• Immunoglobulins exist as membrane-bound receptors on B cells, triggering clonal expansion upon antigen binding, and as secreted antibodies that neutralize pathogens and interact with innate immune cells via the Fc region.
  Related references: PMID:17576170 PMID:20176268 PMID:22158414
• The antigen-binding site is formed by variable domains, while the constant region defines the isotype; IGHG1 specifically mediates IgG1 effector functions such as antibody-dependent cellular cytotoxicity and complement activation.
  Related references: PMID:17576170 PMID:20176268 PMID:22158414
• Subcellularly, IGHG1 is either secreted or anchored on the cell membrane as part of the B cell receptor.
• IGHG1 is heavily modified by glycosylation and disulfide bond formation, which are essential for its stability and function.
• As a member of the immunoglobulin superfamily, IGHG1 plays a crucial role in adaptive immunity and is a common target in diagnostic and therapeutic applications.

Experimental Guidance and Technical Tips

• The immunogen for this antibody is a recombinant Fc fragment, suggesting specificity for the Fc portion of human IgG1. Consider using it to detect IgG1-expressing B cells or antibody-secreting cells in flow cytometry.
• Validate the PE conjugate in your sample system (e.g., PBMCs, serum) with appropriate positive and negative controls.
• Perform antibody titration to optimize staining index and minimize background for flow cytometry assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, supporting diagnostic manufacturers, laboratories, and research institutes from concept to clinical application. This PE-conjugated anti-human IgG Fc monoclonal antibody is a valuable tool for humoral immunity research and flow cytometry-based diagnostic assays. Our portfolio also includes validated antibody pairs, bulk ancillary reagents, and rare target raw-material sourcing to streamline your assay development.