PE Rabbit anti-Human IgA1 Monoclonal Antibody for Flow Cytometry - P01876

Core Product Specifications and Parameters

Biological Background: IgA1 Function and Localization

• Immunoglobulin heavy constant alpha 1 (Ig alpha-1 chain C region) is the constant region of the IgA1 heavy chain, encoded by the IGHA1 gene. It is a major immunoglobulin subclass in humans, existing as both secreted and membrane-bound isoforms.
• As part of IgA antibodies, the constant region mediates humoral immunity. Membrane-bound IgA serves as the B cell receptor, triggering B cell activation upon antigen binding. Secreted IgA participates in the elimination of antigens. Related references: PMID:20176268 PMID:22158414
• The antigen binding site is formed by the variable domain of one heavy chain paired with a light chain. Diversity is generated through V(D)J rearrangement, and affinity maturation occurs via somatic hypermutation after antigen exposure. Related references: PMID:17576170 PMID:20176268
• IgA1 is the predominant immunoglobulin in body secretions such as saliva, tears, and mucosal surfaces, providing first-line immune defense. Related references: PMID:2241915
• Alternative splicing produces both secreted and membrane-bound forms of IgA1. The secreted form is found in bodily fluids, while the membrane-bound form is displayed on the surface of B lymphocytes.
• The protein is a glycoprotein with intrachain disulfide bonds and an immunoglobulin domain structure. Key associated keywords include adaptive immunity, secreted, cell membrane, and glycoprotein.

Experimental Guidance and Technical Tips

• Use this PE-conjugated antibody for direct flow cytometry detection of human IgA1 on B cell surfaces or in intracellular staining after permeabilization.
• The immunogen (amino acids 99-353 of the constant region) ensures subclass specificity; validate absence of cross-reactivity with other immunoglobulin isotypes in your sample system.
• For optimal FC results, titrate the antibody and include appropriate isotype controls and Fc blocking steps.
• Protect from light due to PE conjugation; use freshly prepared samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers this PE Rabbit anti-Human IgA1 monoclonal antibody as a reliable raw material for IVD assay development targeting humoral immunity and immunoglobulin profiling. Our one-stop platform provides diagnostic manufacturers with validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and support for sourcing rare targets—helping to streamline your workflow from concept to clinic.