PE Rabbit anti-Human IFN-gamma Monoclonal Antibody for FC (intra) - P01579

Core Product Specifications and Parameters

Biological Background: Interferon gamma Function and Localization

• Interferon gamma (IFN-gamma) is a type II interferon produced by immune cells, including activated T lymphocytes and NK cells, that orchestrates antimicrobial, antiviral, and antitumor immune responses (PubMed:16914093, PubMed:8666937). Related references: PMID:16914093 PMID:8666937

• IFNG binding to its receptor IFNGR1 triggers the JAK-STAT signaling cascade, resulting in STAT1 activation, nuclear translocation, and transcriptional regulation of target genes (PubMed:8349687). Related references: PMID:8349687

• It plays a critical role in the class I antigen presentation pathway by inducing a switch from constitutive proteasome subunits to immunoproteasome subunits and upregulating the proteasome activator PA28, thereby enhancing peptide generation for MHC I loading (PubMed:8666937, PubMed:8163024, PubMed:11112687). Related references: PMID:8666937 PMID:8163024 PMID:11112687

• IFN-gamma upregulates MHC II complexes on the cell surface by inducing expression of cathepsins B, H, and L (PubMed:7729559). Related references: PMID:7729559

• Many of the immediate early genes induced by IFN-gamma are transcription factors such as IRF1, which further propagate the transcriptional response (PubMed:16914093). Related references: PMID:16914093

• IFN-gamma also participates in the regulation of hematopoietic stem cells, influencing their development, quiescence, and differentiation during homeostasis (By similarity).

• The protein is secreted and post-translationally modified; key modifications include glycosylation, cleavage on basic residues, and formation of pyrrolidone carboxylic acid, with a molecular weight of approximately 19 kDa.

Experimental Guidance and Technical Tips

• This PE-conjugated monoclonal antibody is ready-to-use for intracellular flow cytometry (FC intra). Consider appropriate stimulation (e.g., PMA/ionomycin) and fixation/permeabilization protocols for detecting IFN-gamma in activated human T cells or NK cells.
• Optimal working dilution should be determined experimentally by the end user; a typical starting point for flow cytometry is 0.5–5 µL per test, but titration is recommended.
• Cross-reactivity is restricted to human; if working with non-human primate samples, validate cross-reactivity independently due to potential epitope differences.
• For research use only; not yet validated for diagnostic or clinical applications; commercial diagnostic developers should conduct their own performance and stability studies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, laboratories, and research institutes a streamlined procurement pathway for IVD raw materials, including validated monoclonal antibodies, bulk ancillary reagents, and rare target reagents. This PE Rabbit anti-Human IFN-gamma mAb is part of our comprehensive immunology portfolio, supporting developers in immunoassay and flow cytometry-based applications. From antibody pair screening to scale-up supply, CamelBio helps you bridge the gap from concept to clinic with reliable raw material solutions.