PE Rabbit anti-Human ICAM-1/CD54 Monoclonal Antibody for FC - P05362

Core Product Specifications and Parameters

Biological Background: ICAM-1/CD54 Function and Localization

• ICAM-1 acts as a ligand for LFA-1 (ITGAL:ITGB2), ensuring leukocyte cell-cell adhesion and calibrating T-cell cytotoxicity in response to antigen stimulation strength (PubMed:3086451, PubMed:3340213, PubMed:38195629). Related references: PMID:3086451, PMID:3340213, PMID:38195629
• Also functions as a ligand for Mac-1 (ITGAM:ITGB2), mediating adhesion between stimulated neutrophils and endothelial cells (PubMed:1980124). Related references: PMID:1980124
• During leukocyte trans-endothelial migration, ICAM-1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation (PubMed:17875742). Related references: PMID:17875742
• Promotes cell aggregation in epithelial cells through interaction with MUC1 (PubMed:11173916). Related references: PMID:11173916
• Serves as a receptor for major group rhinoviruses and Coxsackievirus A21, mediating viral entry.
• Upon HHV-8 infection, ICAM-1 is degraded by viral E3 ligase MIR2, potentially preventing immune clearance.
• Expressed on vascular endothelial cells, fibroblasts, and hematopoietic cells like macrophages and stimulated T lymphocytes; low expression on resting leukocytes (PubMed:3086451). Related references: PMID:3086451
• Subcellular localization: primarily cell membrane, with a signal peptide, extracellular immunoglobulin-like domains, a transmembrane segment, and a short cytoplasmic tail.
• Post-translational modifications include glycosylation, phosphorylation, and ubiquitination; contains disulfide bonds and repeat regions.

Experimental Guidance and Technical Tips

• This PE-conjugated antibody is designed for flow cytometry (FC); validate in ICAM-1-expressing cell lines such as endothelial cells or stimulated leukocytes.
• Use appropriate isotype controls and single-color compensation when analyzing multicolor panels.
• Protect the antibody from light during staining and storage to preserve PE fluorescence.
• Titrate the antibody to determine the optimal concentration for your specific experimental system.
• Consider including a viability dye to exclude dead cells and improve gating accuracy.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with streamlined access to validated IVD raw materials, including this PE-labeled anti-ICAM-1/CD54 monoclonal antibody for applications in inflammation monitoring, immunotherapy research, and viral receptor studies. Our portfolio encompasses monoclonal/polyclonal antibodies, antibody pairs, and ancillary reagents, with custom sourcing for rare targets and bulk supply options to accelerate your assay development.