PE Rabbit anti-Human HMOX1 mAb - P09601

Core Product Specifications and Parameters

Biological Background: HMOX1 Function and Localization

• HMOX1 (heme oxygenase 1), also known as HO-1 or HSP32, is a ~33 kDa enzyme encoded by the HMOX1 gene (GeneID: 3162). It belongs to the heme oxygenase family.
• Catalyzes the rate-limiting step in heme degradation, cleaving the heme ring at the alpha-methene bridge to yield equimolar amounts of biliverdin IXalpha, carbon monoxide (CO), and ferrous iron (PubMed:11121422, PubMed:19556236, PubMed:7703255). Related references: PMID:11121422, PMID:19556236, PMID:7703255
• Provides cytoprotection against oxidative stress and apoptosis by catabolizing pro-oxidant free heme, thereby preventing heme-mediated sensitization of cells to programmed cell death (PubMed:20055707). Related references: PMID:20055707
• During SARS-CoV-2 infection, HMOX1 is implicated in promoting ORF3A-mediated autophagy, though its role in reticulophagy is unclear.
• Localizes predominantly to the endoplasmic reticulum membrane, consistent with its role in heme detoxification and cellular iron homeostasis.
• Post-translational modifications include phosphorylation, and the protein contains a transmembrane helix anchoring it to the ER membrane.
• Expressed at higher levels in renal cancer tissue compared to normal tissue, indicating potential relevance in tumor biology.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 1-265 of human HMOX1 (NP_002124.1), encompassing the majority of the protein; this design may enable recognition of both native and denatured HMOX1, supporting intracellular flow cytometry and potentially other applications.
• For intracellular flow cytometry, optimize fixation and permeabilization conditions to ensure accessibility of the epitope, as HMOX1 is membrane-anchored at the ER.
• Validate antibody specificity in the relevant sample system, such as heme-treated or stressed cells, to confirm induction of HMOX1 expression.
• Consider using appropriate negative controls (e.g., untreated cells or knockout lines) to assess background staining.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a trusted one-stop sourcing partner for IVD raw materials, serving diagnostic manufacturers, laboratories, and research institutes worldwide. This anti-HMOX1 antibody supports research into oxidative stress, apoptosis, and viral pathogenesis—areas where reliable detection reagents are critical. In addition to validated monoclonal antibodies like this one, CamelBio offers optimized antibody pairs, bulk ancillary reagents, and custom sourcing for rare targets, streamlining your path from concept to clinic.