PE Rabbit anti-Human HIF-1 alpha Monoclonal Antibody for FC (intra) - Q16665

Core Product Specifications and Parameters

Biological Background: HIF-1 alpha Function and Localization

• HIF-1 alpha functions as a master transcriptional regulator of the adaptive response to hypoxia, activating over 40 genes including erythropoietin, VEGF, glycolytic enzymes, and glucose transporters to increase oxygen delivery and facilitate metabolic adaptation. Related references: PMID:11292861 PMID:11566883 PMID:15465032
• It heterodimerizes with ARNT and the complex binds to the hypoxia response element (HRE) in target gene promoters. Transcriptional activation requires recruitment of coactivators such as CREBBP and EP300. Related references: PMID:16543236 PMID:9887100
• HIF-1 alpha activity is enhanced by interaction with coactivators NCOA1 and NCOA2, and the redox regulator APEX1 further potentiates activation. Related references: PMID:10594042 PMID:10202154
• Plays an essential role in embryonic vascularization, tumor angiogenesis, and the pathophysiology of ischemic disease. Related references: PMID:22009797
• Involved in the axonal distribution and transport of mitochondria in neurons during hypoxia. Related references: PMID:19528298
• Upon SARS-CoV-2 infection, HIF-1 alpha is required for induction of glycolysis in monocytes, promoting a pro-inflammatory state and enhancing viral replication. Related references: PMID:32697943
• Localizes to the cytoplasm, nucleus, and nuclear speckles, depending on oxygen levels.
• Expressed in most tissues with highest levels in kidney and heart; frequently overexpressed in human cancers due to intratumoral hypoxia.
• Undergoes extensive post-translational modifications including hydroxylation, acetylation, phosphorylation, and ubiquitination, which regulate its stability and activity. Subject to alternative splicing, producing isoforms with distinct activities.

Experimental Guidance and Technical Tips

• The immunogen is an internal peptide (aa 401-500); ensure permeabilization for intracellular flow cytometry staining.
• Validate antibody performance in relevant human cell lines under hypoxic versus normoxic conditions to confirm hypoxia-inducible expression.
• Titrate the antibody to determine the optimal concentration for FC (intra) to minimize background and maximize signal.
• Use appropriate isotype controls and compensation controls when performing multicolor flow cytometry.
• Consider testing on HIF-1 alpha knockout or knockdown cells as a specificity control.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with comprehensive access to IVD raw materials, including validated monoclonal antibodies like this PE-conjugated anti-HIF-1 alpha antibody. We support procurement of matched antibody pairs, optimized bulk reagents, and rare target raw materials for hypoxia and cancer research applications. Partner with CamelBio to streamline your supply chain from concept to clinic.