PE Rabbit anti-Human GPRC5D Monoclonal Antibody for Flow Cytometry - Q9NZD1

Core Product Specifications and Parameters

Biological Background: GPRC5D Function and Localization

• GPRC5D belongs to the G protein-coupled receptor family C group 5, functioning as a cell membrane receptor involved in the development of hair and nails by regulating hard keratin expression.
• The gene encoding human GPRC5D is located on chromosome 12 and is subject to alternative splicing, producing variants that may influence protein function or localization.
• GPRC5D is widely expressed in the peripheral system, with high expression in pancreas, medium expression in kidney, small intestine, spleen, and testis, and low or undetectable levels in brain, heart, liver, and other tissues.
• At the subcellular level, GPRC5D is localized to the cell membrane, consistent with its role as a transmembrane signal transducer.
• Structural studies (3D-structure available) reveal that GPRC5D contains seven transmembrane helices, typical of GPCRs, and features an N-terminal extracellular domain likely involved in ligand binding.
• This receptor has been identified through proteomics approaches and is classified as a transducer with reference proteome status, underscoring its biological relevance.
• While primarily associated with keratinization, GPRC5D may also participate in other signaling pathways given its broad tissue distribution and GPCR nature.

Experimental Guidance and Technical Tips

• The immunogen used to generate this antibody corresponds to the N-terminal region (amino acids 1-100) of GPRC5D. Researchers should verify that the epitope is accessible in their experimental system, particularly if studying cleaved or modified forms of the receptor.
• This PE-conjugated antibody is validated for flow cytometry (FC). When designing FC experiments, consider using appropriate isotype controls and compensation controls to ensure accurate detection of GPRC5D surface expression.
• Given the reported molecular weight range (34–38 kDa), confirm the observed band in your samples is consistent with the expected size, possibly accounting for glycosylation or other post-translational modifications that may shift the apparent weight.
• For tissues with low GPRC5D expression (e.g., lung, colon), optimization of staining protocols, such as increasing antibody concentration or using signal amplification, may be necessary.
• If performing multiplex FC, confirm that PE fluorescence does not overlap with other fluorophores in your panel, and titrate the antibody to achieve optimal signal-to-noise ratio.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers, laboratories, and research institutes by providing reliable access to IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. For studies focused on GPCR signaling, keratinization disorders, or other areas involving GPRC5D, our integrated supply chain can source rare-target raw materials to accelerate your development pipeline. Let CamelBio be your partner in bridging the gap from concept to clinic.