PE Rabbit anti-Human FGFR3 Monoclonal Antibody for Flow Cytometry - P22607

Core Product Specifications and Parameters

Biological Background: FGFR3 Function and Localization

• FGFR3 is a cell-surface tyrosine kinase receptor for fibroblast growth factors, controlling proliferation, differentiation, and apoptosis.
• It is essential for chondrocyte differentiation and proper skeletal development; gain-of-function mutations cause achondroplasia and related dwarfisms.
• Ligand binding triggers autophosphorylation and activates downstream signaling via FRS2, leading to RAS-MAPK and AKT pathways; PLCG1 phosphorylation also occurs.
• Alternative splicing generates multiple isoforms, including a secreted isoform 3 that binds FGFs and can antagonize signaling.
• The full-length receptor localizes primarily to the cell membrane, with additional pools in cytoplasmic vesicles and the endoplasmic reticulum; the secreted isoform is extracellular.
• Post-translational modifications include glycosylation, disulfide bonds stabilizing its immunoglobulin-like domains, and phosphorylation as part of kinase activation; ubiquitination regulates receptor degradation.
• High expression is observed in brain, kidney, and testis, while expression is low in spleen, heart, and muscle; fetal tissues show elevated levels in kidney, lung, intestine, and brain.
• Diseases: Activating FGFR3 mutations are linked to skeletal dysplasias, craniosynostosis, and deafness; overexpression or constitutive activation is seen in bladder cancer, multiple myeloma, and other malignancies.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (a.a. 23-375), suggesting the antibody recognizes an epitope on the cell surface, compatible with live-cell flow cytometry.
• For FC, use FGFR3-positive human cell lines (e.g., RT4 bladder carcinoma) and include appropriate isotype controls; titrate the antibody to determine optimal staining concentration.
• Assess cross-reactivity with other FGFR family members if multiplexing; confirm specificity with knockout or knockdown validation in your system.
• Fixation and permeabilization may enable detection of intracellular receptor pools but could alter epitope accessibility; evaluate fixation protocols carefully.
• Be aware of multiple isoforms; select validated reference cell lines or recombinant protein controls to verify isoform recognition if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports IVD developers with a comprehensive portfolio of raw materials covering every stage from concept to clinic. For FGFR3-targeted diagnostics, we offer validated monoclonal antibodies, matched antigen/antibody pairs, and ancillary reagents to accelerate assay development in oncology and skeletal disorder research. Our one-stop sourcing model ensures seamless access to high-quality, batch-to-batch consistent raw materials tailored to your workflow.