PE Rabbit anti-Human Desmoglein 3 (DSG3) Monoclonal Antibody for Flow Cytometry - P32926

Core Product Specifications and Parameters

Biological Background: Desmoglein-3 Function and Localization

• Desmoglein-3 is a core component of desmosome cell-cell junctions and is required for the positive regulation of cellular adhesion. Related references: PMID:31835537
• It is required for the assembly of adherens and desmosome junctions in response to mechanical force in keratinocytes. Related references: PMID:31835537
• Essential for anchoring telogen hairs in the hair follicle via desmosome-mediated adhesion of cells surrounding the hair club and the basal layer of the outer root sheath epithelium. Related references: PMID:9701552
• May play a role in maintaining the protein stability of desmosome plaque components, including DSP, JUP, PKP1, PKP2, and PKP3. Related references: PMID:22294297
• Required for YAP1 localization at the plasma membrane in keratinocytes under mechanical strain, forming a complex with PKP1 and YWHAG; may also positively regulate YAP1 target gene transcription and cell proliferation. Related references: PMID:31835537
• Positively regulates cellular contractility and cell junction formation by organizing cortical F-actin bundles and anchoring actin to tight junctions, in conjunction with RAC1. Related references: PMID:22796473
• Inhibits keratinocyte migration by suppressing p38MAPK signaling, thereby moderating wound healing. Related references: PMID:26763450
• Subcellularly localized at the cell membrane, desmosomes, tight junctions, and in the cytoplasm.
• Tissue expression includes the basal and spinous layers of the epidermis, skin, mucosa, esophageal mucosa, and the outer root sheath of hair follicles; also detected in tongue and tonsil.
• Functional keywords: calcium-binding, cell adhesion, glycoprotein, transmembrane, tight junction component.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 50–615 of human DSG3, covering the extracellular cadherin repeat domains; this design is likely to generate antibodies that recognize the native protein.
• This PE-conjugated monoclonal antibody is intended for flow cytometry. For optimal results, consider titrating the antibody in the relevant sample system (e.g., human keratinocytes or mucosal epithelial cells) and include appropriate isotype controls.
• Specificity can be verified by parallel staining with an unconjugated anti-DSG3 antibody and secondary detection to distinguish PE-related artifacts.
• As DSG3 is a transmembrane surface protein, live‑cell staining without permeabilization is recommended for detecting cell‑surface desmoglein‑3.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with a one‑stop sourcing solution for IVD raw materials. From validated antibody pairs to optimized monoclonal and polyclonal antibodies and bulk ancillary reagents, we support projects from concept to clinic. For desmoglein‑3 research in autoimmune blistering diseases such as pemphigus vulgaris, we offer rapid access to this PE‑conjugated antibody along with complementary raw materials to accelerate your assay development.