PE Rabbit anti-Human CPT1A Monoclonal Antibody for Intracellular Flow Cytometry - P50416

Core Product Specifications and Parameters

Biological Background: CPT1A Function and Localization

• Protein Identity: CPT1A (Carnitine O-palmitoyltransferase 1, liver isoform) is encoded by the CPT1A gene in humans and is also known as CPT1-L or L-CPT1. It belongs to the carnitine acyltransferase family and localizes to the mitochondrial outer membrane.
• Core Function in Fatty Acid Metabolism: CPT1A catalyzes the transfer of long-chain fatty acyl groups from CoA to carnitine, a rate-limiting step required for mitochondrial import of fatty acids and subsequent beta-oxidation. This function is critical for hepatic triglyceride metabolism and energy homeostasis. Related references: PMID:11350182 PMID:14517221 PMID:16651524
• Additional Enzymatic Activities: Beyond its canonical carnitine acyltransferase activity, CPT1A exhibits lysine succinyltransferase activity, capable of modifying metabolic enzymes such as ENO1. This activity regulates target protein function independently of its role in fatty acid transport. Related references: PMID:29425493
• Role in Immune Regulation: CPT1A participates in inducible regulatory T-cell (iTreg) differentiation once activated by butyryl-CoA, which counteracts malonyl-CoA-mediated repression. Moreover, it sustains the type I interferon response by recruiting ZDHHC4 to palmitoylate MAVS at mitochondria, thereby stabilizing MAVS and promoting antiviral signaling. Related references: PMID:38016475
• Involvement in Oxidative Stress and Liver Injury: In liver injury contexts, CPT1A promotes reactive oxygen species (ROS)-induced oxidative stress through modulation of NFE2L2 and NLRP3-mediated signaling pathways (By similarity).
• Tissue Expression: CPT1A is strongly expressed in kidney and heart, with relatively lower expression in liver and skeletal muscle.
• Post‑translational Modifications and Structural Features: The protein is subject to acetylation, phosphorylation, and nitration. It contains transmembrane helices and is anchored to the mitochondrial outer membrane. Alternative splicing generates multiple isoforms.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 520–720) lies within a conserved region of human CPT1A; consider verifying sequence homology if cross-reactivity with other species is intended.
• Since this is a PE‑conjugated antibody, use appropriate isotype controls and Fc receptor blocking for flow cytometry experiments.
• Intracellular staining (FC intra) requires fixation and permeabilization of cells. Optimize the fixation/permeabilization protocol based on your cell type and target epitope accessibility.
• CPT1A is a mitochondrial membrane protein; ensure that permeabilization conditions sufficiently expose the epitope for detection.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with a complete range of IVD raw materials, from validated antibody pairs and optimized monoclonal antibodies to bulk ancillary reagents and rare‑target sourcing. With a focus on lipid metabolism and mitochondrial research, we help streamline development pipelines by offering products like this PE‑conjugated CPT1A antibody alongside complementary tools for assay construction and biomarker discovery. Whatever your raw‑material needs may be, CamelBio is ready to support you from concept to clinic.