PE Rabbit anti-Human CD96/TACTILE mAb for FC - P40200

Core Product Specifications and Parameters

Biological Background: CD96/TACTILE Function and Localization

• T-cell surface protein tactile (CD96, TACTILE) is a type I membrane protein belonging to the immunoglobulin superfamily, functioning as a cell adhesion molecule.
• It plays a role in adhesive interactions of activated T and NK cells during the late phase of immune response, promoting NK cell-target adhesion via interaction with PVR (CD155) on target cells.
• CD96 is expressed on normal T-cell lines and clones, some transformed T-cells, and at very low levels on activated B cells; it is not detected on other cultured cell lines.
• The protein localizes to the plasma membrane, consistent with its role in cell-cell adhesion and immune surveillance.
• Sequence analysis predicts the presence of immunoglobulin-like domains, and the protein is likely glycosylated with disulfide bonds, as indicated by UniProt keywords.
• Gene ontology annotations link CD96 to cell adhesion, and clinical keywords associate it with craniosynostosis and chromosomal rearrangements, though its direct involvement in these conditions requires further study.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 25-519, corresponding to the extracellular region of CD96; the antibody may recognize native and recombinant forms.
• For flow cytometry, titrate the antibody to determine the optimal concentration for your specific cell system. Include appropriate isotype controls and viability staining.
• Since the antibody is PE-conjugated, protect from prolonged light exposure and handle as per standard fluorochrome-conjugated antibody protocols.
• Validate staining on known CD96-expressing cells (e.g., activated T cells or NK cell lines) and consider using PVR-blocking experiments to confirm specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw-material procurement for diagnostic developers and research labs, offering a one-stop platform for validated antibodies, bulk reagents, and rare-target sourcing. This PE anti-CD96 monoclonal antibody supports immunophenotyping and T/NK cell research. For projects requiring additional antibody pairs, custom conjugations, or ancillary reagents, CamelBio’s supply network can assist in bridging your sourcing needs.