PE Rabbit anti-Human CD9 Monoclonal Antibody for Flow Cytometry - P21926

Core Product Specifications and Parameters

Biological Background: CD9 Function and Localization

• CD9 antigen is a member of the tetraspanin family, also known as Tetraspanin-29 (Tspan-29), motility-related protein MRP-1, and p24, encoded by the CD9 gene.
• It is an integral membrane protein that regulates key processes including sperm-egg fusion, platelet activation and aggregation, and cell adhesion (PubMed:14575715, PubMed:18541721, PubMed:8478605). Related references: PMID:14575715, PMID:18541721, PMID:8478605
• CD9 inhibits cell fusion events: it prevents myotube fusion during muscle regeneration, macrophage fusion into multinucleated giant cells (PubMed:12796480), and osteoclast formation. Related references: PMID:12796480
• Plays a role in cell adhesion, cell motility, and tumor metastasis (PubMed:7511626, PubMed:8478605). Related references: PMID:7511626, PMID:8478605
• Localizes to the cell membrane and is secreted via extracellular exosomes; it is a transmembrane protein with four transmembrane domains.
• Undergoes post-translational modifications including palmitoylation, glycosylation, and contains disulfide bonds, classifying it as a glycoprotein and lipoprotein.
• Expressed in platelets (at protein level) and a variety of hematopoietic and epithelial cells (PubMed:19640571). Related references: PMID:19640571
• Keywords: Fertilization, Cell adhesion, Tetraspanin, Transmembrane helix.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the full-length human CD9 protein (aa 1-228; NP_001760.1), suggesting that the antibody targets native conformational epitopes on the cell surface.
• Recommended for flow cytometry (FC) applications; since the antibody is PE-conjugated, it can be used for direct detection without secondary reagents.
• Titrate the antibody to determine the optimal concentration for your specific experimental setup to minimize background and ensure signal specificity.
• Include appropriate isotype controls and live/dead cell exclusion to accurately gate CD9-positive populations.
• CD9 is expressed on a wide range of hematopoietic and epithelial cells; use antigen-specific verification (e.g., blocking with recombinant CD9 protein) to confirm specificity in your target cell types.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your strategic partner for IVD raw-material procurement, offering a comprehensive portfolio spanning validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. This anti-CD9 antibody serves as a powerful tool for researchers investigating cell surface markers, tetraspanin biology, and immune cell profiling. Our dedicated sourcing service ensures seamless access to rare targets and custom reagent development, supporting your projects from concept to clinic.