PE Rabbit anti-Human CD8b Monoclonal Antibody for Flow Cytometry - P10966

Core Product Specifications and Parameters

Biological Background: CD8b Function and Localization

• CD8b, also known as CD8 beta chain, is a T-cell surface glycoprotein belonging to the immunoglobulin superfamily. It exists in multiple isoforms (isoform 1, 3, 5, 6, 7, 8) generated by alternative splicing.
• CD8b forms heterodimers with CD8 alpha chain and functions as a coreceptor for MHC class I:peptide complexes on antigen-presenting cells.
• Upon binding to MHC class I, CD8b recruits the Src kinase LCK to the TCR-CD3 complex, initiating intracellular signaling cascades that promote T-cell activation, proliferation, and cytotoxic function.
• The cytoplasmic tail of CD8b contains a palmitoylation site that targets the protein to lipid rafts, concentrating signaling molecules and enhancing signal transduction efficiency.
• CD8b is critical for thymic selection of CD8+ T-cells, ensuring proper development of the cytotoxic T-lymphocyte repertoire.
• Subcellularly, CD8b localizes primarily to the plasma membrane, but secreted isoforms have also been detected.
• Tissue expression is restricted to thymus and peripheral CD8+ T-cells, with certain isoforms showing preferential expression in thymic versus peripheral populations.
• Post-translational modifications include N-linked glycosylation, disulfide bond formation, and phosphorylation, which may regulate protein stability and interaction properties.

Experimental Guidance and Technical Tips

• The immunogen was designed against the extracellular domain (aa 22-170) of human CD8b, encompassing the immunoglobulin-like V-type domain critical for MHC I interaction, which makes it suitable for detecting surface-expressed CD8b by flow cytometry.
• As this antibody is validated for flow cytometry, consider using it for immunophenotyping of T-cell subsets, but perform titration experiments to determine the optimal concentration.
• Since the antibody is directly conjugated to PE (phycoerythrin), ensure proper compensation settings in multicolor flow cytometry panels, especially when co-staining with FITC or APC.
• For other applications such as Western blotting or immunohistochemistry, the antibody may require re-validation, as the PE conjugate and antigen recognition may behave differently.
• Use appropriate isotype controls and viability dyes in flow cytometry experiments to discriminate specific CD8b staining from background.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive one-stop sourcing bridge for diagnostic manufacturers and researchers, offering validated antibody pairs, optimized monoclonal antibodies like this PE‑conjugated anti‑CD8b mAb, and bulk ancillary reagents for immunology and T‑cell research applications. With a focus on IVD raw‑material supply, we also assist in sourcing rare‑target antibodies and provide technical consultation to accelerate your assay development from concept to clinic.