PE Rabbit anti-Human CD85j/ILT2 (LILRB1) Monoclonal Antibody for Flow Cytometry - Q8NHL6

Core Product Specifications and Parameters

Biological Background: LILRB1 (CD85j/ILT2) Function and Localization

• LILRB1 (CD85j/ILT2) is an inhibitory receptor belonging to the leukocyte immunoglobulin-like receptor (LILR) family, containing immunoglobulin-like domains.
• It functions as a receptor for class I major histocompatibility complex (MHC) antigens, recognizing a broad spectrum including HLA-A, HLA-B, HLA-C, HLA-G, and HLA-F alleles. Related references: PMID:16455647 PMID:28636952
• Additionally recognizes the cytomegalovirus class I MHC homolog UL18, inhibiting immune responses.
• Engagement of LILRB1 on natural killer cells or T cells by class I MHC molecules protects target cells from lysis. Interaction with HLA-B or HLA-E leads to inhibition of FCER1A signaling and serotonin release, and suppresses FCGR1A-mediated phosphorylation and calcium mobilization. Related references: PMID:11907092 PMID:9285411 PMID:9842885
• Recognizes HLA-G complexed with beta-2-microglobulin (B2M) and a nonamer self-peptide, triggering secretion of growth-promoting factors by decidual NK cells. Related references: PMID:19304799 PMID:29262349
• Reprograms B cells toward an immune suppressive phenotype. Related references: PMID:24453251
• Subcellularly localized to the cell membrane and also secreted.
• Expressed in B cells, monocytes, various dendritic cell subsets, decidual macrophages, and decidual NK cells.
• Post-translational modifications include glycosylation and phosphorylation, contributing to observed molecular weights of 49–71 kDa.

Experimental Guidance and Technical Tips

• The immunogen used was a recombinant fusion protein encompassing amino acids 24–458, covering the extracellular region. For flow cytometry, this antibody recognizes a cell surface epitope; ensure appropriate cell preparation and use of live-cell staining protocols.
• Due to the expression pattern, this antibody is suitable for identifying LILRB1-positive populations in human peripheral blood mononuclear cells (PBMCs) and immune cell subsets.
• The multiple molecular weights (49–71 kDa) reflect glycosylation variants; consider this when including a total protein control.
• For optimal staining, titrate the antibody to determine the best concentration for your specific cell type and experimental conditions.
• Note that the PE conjugate is light-sensitive; handle stained samples in the dark and acquire promptly.

CamelBio: Your One-Stop Sourcing Bridge

As a specialized supplier of IVD raw materials, CamelBio provides diagnostic manufacturers and research laboratories with reliable access to validated monoclonal and polyclonal antibodies, including antibody pairs, bulk reagents, and rare target raw materials. This PE-conjugated anti-human LILRB1 antibody supports immunology and immuno-oncology research applications, with additional sourcing options available for ancillary reagents and customized assay development.