PE Rabbit anti-Human CD85d/e/i/j Monoclonal Antibody for FC - Q8N423 / Q8N6C8 / O75019 / Q8NHL6

Core Product Specifications and Parameters

Biological Background: CD85d (LILRB2) Function and Localization

• Leukocyte immunoglobulin-like receptor subfamily B member 2 (LILRB2), also known as CD85d, ILT-4, LIR-2, and MIR-10, is an inhibitory receptor primarily expressed on myeloid cells and a member of the leukocyte immunoglobulin-like receptor (LIR) family.
• Serves as a receptor for class I MHC antigens, recognizing a broad spectrum of HLA-A, HLA-B, HLA-C, HLA-G, and HLA-F alleles, thereby contributing to immune down-regulation and the development of tolerance. Related references: PMID:11169396 PMID:12853576 PMID:16455647
• Recognizes HLA-G in complex with B2M and a nonamer self-peptide, triggering differentiation of type 1 regulatory T cells and myeloid-derived suppressor cells, both of which actively maintain maternal-fetal tolerance. Related references: PMID:16455647 PMID:20448110 PMID:27859042
• Competes with CD8A for binding to class I MHC antigens and inhibits FCGR1A-mediated phosphorylation of cellular proteins and mobilization of intracellular calcium ions. Related references: PMID:11875462 PMID:12853576 PMID:9548455
• Is a single-pass type I membrane protein localized to the cell membrane and contains several immunoglobulin-like domains, disulfide bonds, and glycosylation sites.
• Expressed in monocytes, myeloid and plasmacytoid dendritic cells, tolerogenic IL-10-producing dendritic cells, myeloid-derived suppressor cells during pregnancy, and at low levels in natural killer cells and B cells.
• Subject to alternative splicing, alternative promoter usage, and phosphorylation, which may modulate its function and expression.

Experimental Guidance and Technical Tips

• The immunogen used corresponds to the extracellular domain (aa 22–461) of human CD85d, suggesting the antibody likely recognizes epitopes within this region; consider validating reactivity against native CD85d on relevant cell lines.
• Since the antibody cross-reacts with CD85e, CD85i, and CD85j, careful experimental design (e.g., blocking with recombinant proteins or using knockout/knockdown models) is recommended to confirm target specificity in flow cytometry.
• For flow cytometry, titrate the antibody to determine the optimal concentration for your specific sample type and staining conditions; PE conjugation is compatible with common compensation setups when using multicolor panels.
• The antibody is supplied on an ice bag; upon receipt, store at 2–8°C and protect from light to preserve fluorophore stability.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with streamlined access to IVD raw materials, technical services, and consulting—supporting every stage from concept to clinic. This PE-conjugated anti-CD85d/e/i/j monoclonal antibody is a valuable tool for studying immune regulation, maternal-fetal tolerance, and checkpoint pathways. Beyond antibodies, CamelBio offers validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and sourcing solutions for rare targets, making it a trusted partner for your IVD development needs.