PE Rabbit anti-Human CD82 Monoclonal Antibody for FC - P27701

Core Product Specifications and Parameters

Biological Background: CD82 Function and Localization

• CD82 is a structural component of tetraspanin-enriched microdomains (TERMs), platforms for receptor clustering and signaling. Related references: PMID:19497983
• Modulates EGF signaling by facilitating ligand-induced endocytosis of EGFR and its subsequent desensitization, and regulates EGFR ubiquitination via CBL phosphorylation. Related references: PMID:10985391 | PMID:35538033 | PMID:23897813
• Increases cell-matrix adhesion by regulating membrane organization of integrin alpha4/ITA4. Related references: PMID:24623721 | PMID:8757325
• Suppresses cell migration through other integrins such as alpha6/ITGA6 and beta1/ITGB1. Related references: PMID:15557282 | PMID:17560548
• Decreases cell-associated plasminogen activation by interfering with the interaction between urokinase-type plasminogen activator (PLAU) and its receptor PLAUR. Related references: PMID:15677461
• Inhibits LPS-induced inflammatory response by preventing binding of LPS to TLR4 on the cell surface. Related references: PMID:36945827
• Controls angiogenesis by sequestering VEGFA and PDGFB to inhibit their signaling. Related references: PMID:34530889
• Subcellularly localized to the cell membrane, cytoplasmic vesicles, and phagosomes.
• Lymphoid-specific expression; post-translationally modified by glycosylation and palmitoylation.

Experimental Guidance and Technical Tips

• This antibody is PE-conjugated, making it directly suitable for flow cytometry without the need for a secondary detection step. Consider titrating the antibody to determine the optimal concentration for your specific cell type and staining conditions.
• CD82 is expressed predominantly in lymphoid cells; validate the antibody in appropriate human lymphoid cell lines or primary cells.
• For fixation/permeabilization protocols, test whether the PE conjugate withstands the required conditions, as some fluorophores are sensitive to harsh treatments.
• Use appropriate isotype controls and live/dead discrimination to ensure accurate analysis.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with one-stop access to IVD raw materials, including validated monoclonal antibodies like this anti-CD82 clone. We support your tetraspanin and cancer metastasis research with bulk primary antibodies, optimized antibody pairs, and ancillary reagents. For rare or emerging targets, our sourcing experts can help secure difficult-to-find raw materials, streamlining your development from concept to clinic.